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Monoclonal antibodies: magic bullets with a hefty price tag

Allen F Shaughnessy1

  • 1Tufts University School of Medicine, Boston, Massachusetts, USA. Allen.Shaughnessy@Tufts.edu

BMJ (Clinical Research Ed.)
|December 14, 2012
PubMed
Abstract

No abstract available in PubMed .

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Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...