Flow-dependent double-nanohole optical trapping of 20 nm polystyrene nanospheres

We study the influence of fluid flow on the ability to trap optically a 20 nm polystyrene particle from a stationary microfluidic environment and then hold it against flow. Increased laser power is required to hold nanoparticles as the flow rate is increased, with an empirical linear dependence of 1 μl/(min×mW). This is promising for the delivery of additional nanoparticles to interact with a trapped nanoparticle; for example, to study protein-protein interactions, and for the ability to move the trapped particle in solution from one location to another.