Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
DNA Isolation01:34

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular and Biochemical Analysis of Chalcone Synthase from Freesia hybrid in flavonoid biosynthetic pathway.

PloS one·2015
Same author

Apolipoprotein ε4 is associated with lower brain volume in cognitively normal Chinese but not white older adults.

PloS one·2015
Same author

Accumulation of a bioactive benzoisochromanequinone compound kalafungin by a wild type antitumor-medermycin-producing streptomycete strain.

PloS one·2015
Same author

Protease nexin 1 induces apoptosis of prostate tumor cells through inhibition of X-chromosome-linked inhibitor of apoptosis protein.

Oncotarget·2015
Same author

Inhibition of hepatitis B virus gene expression and replication by hepatocyte nuclear factor 6.

Journal of virology·2015
Same author

Protein tyrosine phosphatase receptor type O expression in the tumor niche correlates with reduced tumor growth, angiogenesis, circulating tumor cells and metastasis of breast cancer.

Oncology reports·2015
Same journal

[Physiological function of membrane protein RHOGL009301 involved in transport of benzoate in Rhodococcus sp. R04].

Wei sheng wu xue bao = Acta microbiologica Sinica·2018
Same journal

[Distribution, structure and sequence alignment, and metagenomics analysis of two nitrite reductases with NO forming].

Wei sheng wu xue bao = Acta microbiologica Sinica·2018
Same journal

[Diversity of microbial community structure in the spermosphere of saline-alkali soil in shandong area].

Wei sheng wu xue bao = Acta microbiologica Sinica·2018
Same journal

[Two sample pooling strategies revealed different root-associated fungal diversity of Rhododendron species].

Wei sheng wu xue bao = Acta microbiologica Sinica·2018
Same journal

[Phylogenetic and genetic heterogeneity of 23 Acidithiobacillus strains isolated from different geographical locations].

Wei sheng wu xue bao = Acta microbiologica Sinica·2018
Same journal

[Effects of glucose on photosynthesis and growth of Chloralla sp. HN08 cells].

Wei sheng wu xue bao = Acta microbiologica Sinica·2018
See all related articles

Related Experiment Video

Updated: May 16, 2026

Extraction of High Molecular Weight Genomic DNA from Soils and Sediments
11:24

Extraction of High Molecular Weight Genomic DNA from Soils and Sediments

Published on: November 10, 2009

[Optimization of soil microbial DNA isolation].

Yudong Zhao1, Jun Zhou, Jing He

  • 1State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. zyd3894816@yahoo.cn

Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica
|December 15, 2012
PubMed
Summary
This summary is machine-generated.

An optimized method isolates high-quality soil microbial DNA, enabling metagenomic studies of uncultured microbes. This technique improves DNA yield, purity, and size for enhanced microbial diversity analysis.

More Related Videos

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere
09:55

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Published on: May 2, 2018

Related Experiment Videos

Last Updated: May 16, 2026

Extraction of High Molecular Weight Genomic DNA from Soils and Sediments
11:24

Extraction of High Molecular Weight Genomic DNA from Soils and Sediments

Published on: November 10, 2009

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere
09:55

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Published on: May 2, 2018

Area of Science:

  • Microbiology
  • Molecular Biology
  • Environmental Science

Background:

  • Over 99% of soil microorganisms are unculturable using conventional methods, limiting our understanding of microbial diversity.
  • The quantity and quality of environmental DNA (eDNA) are critical for successful metagenomic studies.
  • Exploiting uncultured microbial resources is essential for advancing scientific discovery.

Purpose of the Study:

  • To develop and optimize a method for isolating high-quality soil microbial DNA.
  • To enable comprehensive microbial diversity studies and metagenomic library construction.
  • To facilitate the exploration of previously uncultured soil microbial communities.

Main Methods:

  • Compared and optimized key DNA isolation procedures, including cell lysis (SDS-CTAB, lysozyme), protein removal (chloroform), and DNA purification (PVPP column).
  • Evaluated the optimized method against three existing protocols using diverse soil samples.
  • Assessed eDNA yield, purity, size, and PCR amplification efficiency.

Main Results:

  • The optimized method significantly improved soil eDNA quality, yielding 95 µg DNA per gram of soil.
  • Achieved ideal OD A260/A280 and A260/A230 ratios, indicating high purity.
  • Successfully amplified PCR products and obtained eDNA fragments up to 100 kb.

Conclusions:

  • The established method provides high-quality and high-quantity soil microbial DNA.
  • This technique is a powerful tool for metagenomic research, unlocking the potential of uncultured soil microbes.
  • Facilitates deeper insights into soil microbial ecosystems and their functions.