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Published on: July 16, 2013
Possible ATP release through lysosomal exocytosis from primary sensory neurons
Junyang Jung1, Youn Ho Shin, Hiroyuki Konishi
1Department of Anatomy, College of Medicine, Kyung Hee University, Heoki-Dong 1, Dongdaemun-Gu, Seoul 130-701, Republic of Korea.
Abstract:
The adenosine triphosphate (ATP) plays important roles under physiological and pathological conditions such as traumatic brain injury, neuroinflammation and neuropathic pain. In the present study, we set out to study the role of lysosomal vesicles on ATP release from the dorsal root ganglion neurons. We found that the lysosomal vesicles, which contain the quinacrine-positive fluorescence and express the vesicular nucleotide transporter (VNUT), were localized within the soma and growth cone of the cultured dorsal root ganglion neurons. In addition, the number of the quinacrine staining was decreased by application of lysosomal exocytosis activators, and this decrease was suppressed by the metformin and vacuolin-1, which suppressed lysosomal exocytosis. Thus, these findings suggest that ATP release via the lysosomal exocytosis may be one of the pathways for ATP release in response to stimulation.
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