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Lagging Strand Synthesis

Lagging Strand Synthesis

During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...

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Updated: May 16, 2026

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

Multiscale analysis of genome-wide replication timing profiles using a wavelet-based signal-processing algorithm.

Benjamin Audit¹, Antoine Baker, Chun-Long Chen

¹Université de Lyon, Lyon, France. benjamin.audit@ens-lyon.fr

Nature Protocols

|December 15, 2012

Summary

This summary is machine-generated.

This protocol introduces LastWave, an open-source tool for analyzing DNA replication timing. It reveals complex replication patterns by examining fork polarity and initiation zones, offering broader applications than traditional methods.

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Published on: March 22, 2018

Area of Science:

Genomics
Molecular Biology
Computational Biology

Background:

Cellular DNA replication timing is crucial for understanding genome stability and cell cycle regulation.
Existing methods for analyzing replication timing often rely on genome segmentation, which may oversimplify complex patterns.

Purpose of the Study:

To describe a novel protocol using the LastWave open-source signal-processing command language for analyzing cellular DNA replication timing profiles.
To provide a method for interactive visual analysis of DNA replication timing, identifying preferential initiation zones and U-shaped domains.

Main Methods:

Utilizing a multiscale, wavelet-based signal-processing algorithm within the LastWave software.
Analyzing the relationship between timing profiles and fundamental replication program features like fork polarity and origin/termination site density.
Exploring space-scale maps of apparent replication speeds to detect initiation zones.

Main Results:

The LastWave protocol enables interactive visual analysis of DNA replication timing profiles.
It facilitates the detection of preferential replication initiation zones and the delimitation of U-shaped domains.
This approach recognizes more complex spatio-temporal replication patterns compared to traditional genome segmentation.

Conclusions:

LastWave offers a powerful and versatile tool for detailed analysis of DNA replication timing.
The protocol provides a more nuanced understanding of the cell's DNA replication program.
This method has broader applications in genomics and cell biology research.