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Related Experiment Video

Updated: May 16, 2026

Early Viral Entry Assays for the Identification and Evaluation of Antiviral Compounds
09:29

Early Viral Entry Assays for the Identification and Evaluation of Antiviral Compounds

Published on: October 29, 2015

Dioscin's antiviral effect in vitro.

Chaohong Liu1, Yun Wang, Chunchen Wu

  • 1State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071, China. cliu1234@umd.edu

Virus Research
|December 15, 2012
PubMed
Summary

Dioscin, a compound from air potato, shows antiviral potential. It inhibits adenovirus by blocking entry and affects host response, while also inhibiting Hepatitis B virus (HBV) secretion.

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High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
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Published on: May 5, 2014

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Published on: October 29, 2015

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
11:34

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

Published on: May 5, 2014

Area of Science:

  • Natural Product Chemistry
  • Virology
  • Molecular Biology

Background:

  • Dioscin is a natural compound derived from the air potato yam species.
  • Emerging viral infections necessitate the discovery of novel antiviral agents.
  • Understanding the mechanisms of natural compounds against viruses is crucial.

Purpose of the Study:

  • To investigate the antiviral properties of dioscin against adenovirus, vesicular stomatitis virus (VSV), and hepatitis B virus (HBV).
  • To elucidate the mechanism of action of dioscin against adenovirus infection.
  • To evaluate the effect of dioscin on HBV replication markers.

Main Methods:

  • Time-of-addition assays were used to determine the stage of viral infection inhibited by dioscin.
  • Quantitative reverse transcription PCR (qRT-PCR) was employed to measure mRNA levels of the adenovirus receptor (CAR).
  • Enzyme-linked immunosorbent assay (ELISA) was utilized to quantify hepatitis B virus antigens (HBeAg and HBsAg).

Main Results:

  • Dioscin inhibited adenovirus infection by blocking its initial entry and affecting the host cell response.
  • Dioscin treatment led to decreased CAR mRNA levels in host cells, and CAR overexpression restored adenovirus infectivity.
  • Dioscin demonstrated inhibitory effects on VSV infection when cells were pretreated prior to infection.
  • Dioscin reduced the secretion of HBeAg and HBsAg in HBV-positive HepG2 cells.

Conclusions:

  • Dioscin exhibits broad-spectrum antiviral activity, notably against adenovirus, VSV, and HBV.
  • The antiviral mechanism of dioscin against adenovirus involves the downregulation of the CAR receptor.
  • Dioscin represents a potential therapeutic candidate for viral infections, warranting further investigation.