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Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

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Analytical properties of solid-substrate electrospray ionization mass spectrometry.

Bin Hu¹, Pui-Kin So, Zhong-Ping Yao

¹State Key Laboratory of Chirosciences, Food Safety and Technology Research Centre and Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong SAR, China.

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|December 15, 2012

Summary

This study explores using non-capillary emitters for electrospray ionization mass spectrometry (ESI-MS). Wooden tips and other porous materials can serve as effective emitters for ESI-MS analysis.

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Area of Science:

Analytical Chemistry
Mass Spectrometry

Background:

Conventional electrospray ionization mass spectrometry (ESI-MS) relies on capillary emitters.
Ambient ionization techniques are gaining traction, prompting research into non-capillary ESI-MS emitters.

Purpose of the Study:

To investigate and extend the use of non-capillary emitters for ESI-MS.
To explore the potential of wooden tips and other materials as emitters.

Main Methods:

Utilized wooden tips as non-capillary emitters for ESI-MS.
Investigated the chromatographic separation capabilities of wooden tips.
Examined the ionization behavior of proteins and salts on wooden tips.
Tested various non-conductive and sample-derived materials as emitters.

Main Results:

Wooden tips demonstrated chromatographic separation of sample components.
Observed sequential and exhaustive ionization, with salts ionizing before proteins.
Non-conductive materials with microchannels/pores, when loaded with sample solutions, became conductive for ESI-MS.
Direct ESI-MS analysis was achieved using sample materials like tissue, mushroom, and bone as emitters.

Conclusions:

Non-capillary emitters, including wooden tips and other porous materials, are viable for ESI-MS.
These novel emitters offer potential for sample separation and direct analysis.
The findings expand the scope of ESI-MS applications, particularly in ambient ionization settings.