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Published on: September 30, 2019
Selection of antiviral peptides against mink enteritis virus using a phage display Peptide library
Qingming Zhang1, Yuping Wang, Qun Ji
1College of Animal Science and Veterinary Medicine, JiLin University, 5333 Xi'an Road, Changchun, 130062, China. zhangqingming01@126.com
Abstract:
Mink enteritis virus (MEV) causes high morbidity and mortality in mink worldwide, and there are no effective treatments. This study used a phage display library to find specific peptides capable of binding MEV and preventing its replication in F81 cells. After three rounds of biopanning, the phage enrichment was 117 times higher than that after the first round. Twelve phage clones that showed threefold higher MEV-binding affinity than controls were selected by ELISA. Following sequence analyses, the peptides RLNNRARIILRA and LAHKSRLYERHM were synthesized and used for antiviral experiments. MTT assays demonstrated that both peptides increased cell viability by >20 % at 100 μg/ml when pre-incubated with MEV. However, no effect was seen if the peptides were added 2 h after viral inoculation of cells, indicating that the antiviral activity is due to inhibition of viral attachment to the cell surface.
Insights
Researchers identified novel peptides that bind to Mink enteritis virus (MEV), effectively inhibiting its attachment to host cells and preventing viral replication. These findings offer a promising new avenue for treating MEV infections in mink.
Area of Science:
- Virology
- Molecular Biology
- Biotechnology
Background:
- Mink enteritis virus (MEV) poses a significant threat to mink populations globally, causing severe illness and death.
- Current treatment options for MEV are limited, necessitating the development of novel therapeutic strategies.
Purpose of the Study:
- To identify specific peptides that can bind to MEV and inhibit its replication.
- To evaluate the antiviral potential of these peptides against MEV infection in cell culture.
Main Methods:
- A phage display library was employed to screen for MEV-binding peptides.
- Biopanning was performed over three rounds to enrich specific phage clones.
- Enzyme-linked immunosorbent assay (ELISA) and MTT assays were used to assess peptide binding affinity and antiviral activity.
Main Results:
- Phage enrichment increased 117-fold after three rounds of biopanning.
- Twelve phage clones exhibited significantly higher MEV-binding affinity.
- Synthesized peptides RLNNRARIILRA and LAHKSRLYERHM enhanced cell viability by over 20% when pre-incubated with MEV, indicating inhibition of viral attachment.
Conclusions:
- The identified peptides demonstrate potent antiviral activity against MEV by blocking viral attachment to host cells.
- These peptides represent a promising therapeutic candidate for controlling MEV outbreaks in mink.
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