Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Growth of Cartilage and Bone Tissue01:27

Growth of Cartilage and Bone Tissue

Chondrocytes form a temporary cartilaginous model by dividing and secreting a thick gel-like extracellular matrix. Once the chondrocytes undergo programmed cell death, osteoblasts enter the site of the cartilaginous model. The process of replacing the temporary cartilaginous model with bone in an ordered manner is called endochondral ossification. In endochondral ossification, not all of the cartilage is replaced by bone tissue. Some cartilage that performs a protective and supportive function...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A novel knee joint laxity measurement device in mice.

Journal of biomechanics·2026
Same author

STELLAR: A flexible ensemble learning framework integrating rare variants to enhance polygenic risk prediction.

medRxiv : the preprint server for health sciences·2026
Same author

Meniscal Dominant Loaders During Simulated Gait: A Cadaveric Study in Human Knees.

Orthopaedic journal of sports medicine·2026
Same author

Epstein-Barr virus (EBV) infection causes human germinal center B cell-derived lymphomas in the absence of EBNA2 expression.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Integrating common and rare variants improves polygenic risk prediction across diverse populations.

Nature communications·2026
Same author

A human synovial tendon-on-a-chip models key features of peritendinous adhesions and offers a new approach methodology for testing anti-fibrotic drugs.

bioRxiv : the preprint server for biology·2026
Same journal

Polycationic Peptide Engineering of Phage Endolysins Expands Host Range and Enhances Antibacterial and Antibiofilm Activities Against Bacillus Species.

Biotechnology and bioengineering·2026
Same journal

Artificial Intelligence-Powered Algal Biodiesel: The Future of Biofuel Production Through Data-Driven Biotechnology.

Biotechnology and bioengineering·2026
Same journal

Developing Anti-EGFR/Anti-HER2 Bifunctional Antibody for Solid Tumors by Protein Engineering.

Biotechnology and bioengineering·2026
Same journal

Bridging Organ-on-a-Chip and Omics: A Multi-Dimensional Frontier in Biomedical Research.

Biotechnology and bioengineering·2026
Same journal

Hemopexin Purification From Human Cohn Fraction IV Paste and Its Biophysical Characterization and Functional Evaluation in Sickle Cell Disease Mice.

Biotechnology and bioengineering·2026
Same journal

Characterization and Therapeutic Potential of a Novel Lytic Phage-Derived Endolysin PA16cLys Against Uropathogenic Pseudomonas aeruginosa Biofilms.

Biotechnology and bioengineering·2026
See all related articles

Related Experiment Video

Updated: May 16, 2026

Mechanical Stimulation of Chondrocyte-agarose Hydrogels
12:45

Mechanical Stimulation of Chondrocyte-agarose Hydrogels

Published on: October 27, 2012

Engineering superficial zone features in tissue engineered cartilage.

Tony Chen1, Matthew J Hilton, Edward B Brown

  • 1Department of Biomedical Engineering, University of Rochester, Rochester, NY 14642, USA.

Biotechnology and Bioengineering
|December 15, 2012
PubMed
Summary
This summary is machine-generated.

Mimicking joint fluid flow in bioreactors creates a superficial layer in engineered cartilage. This layer enhances matrix production and collagen alignment, crucial for effective cartilage repair.

More Related Videos

Layered Alginate Constructs: A Platform for Co-culture of Heterogeneous Cell Populations
08:57

Layered Alginate Constructs: A Platform for Co-culture of Heterogeneous Cell Populations

Published on: August 7, 2016

Integrated Bone Formation Through In Vivo Endochondral Ossification Using Mesenchymal Stem Cells
06:05

Integrated Bone Formation Through In Vivo Endochondral Ossification Using Mesenchymal Stem Cells

Published on: July 14, 2023

Related Experiment Videos

Last Updated: May 16, 2026

Mechanical Stimulation of Chondrocyte-agarose Hydrogels
12:45

Mechanical Stimulation of Chondrocyte-agarose Hydrogels

Published on: October 27, 2012

Layered Alginate Constructs: A Platform for Co-culture of Heterogeneous Cell Populations
08:57

Layered Alginate Constructs: A Platform for Co-culture of Heterogeneous Cell Populations

Published on: August 7, 2016

Integrated Bone Formation Through In Vivo Endochondral Ossification Using Mesenchymal Stem Cells
06:05

Integrated Bone Formation Through In Vivo Endochondral Ossification Using Mesenchymal Stem Cells

Published on: July 14, 2023

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Orthopedics

Background:

  • Cartilage tissue engineering faces challenges in replicating native tissue's anisotropic extracellular matrix.
  • This anisotropy is vital for mechanical function and biological integration in native cartilage.

Purpose of the Study:

  • To investigate if mimicking synovial fluid flow can induce superficial layer formation in engineered cartilage.
  • To assess the impact of hydrodynamic conditions on matrix production and collagen organization.

Main Methods:

  • Utilized hydrodynamic conditions simulating joint fluid motion in bioreactors.
  • Analyzed engineered cartilage hydrogels for matrix proteoglycan and Type II collagen production.
  • Examined collagen alignment using second harmonic generation imaging.
  • Assessed the expression of surface zone protein Proteoglycan 4 (PRG4).

Main Results:

  • Hydrodynamic flow induced a distinct superficial layer in engineered cartilage.
  • This layer showed enhanced production of proteoglycan and Type II collagen.
  • Flow stimulation upregulated Proteoglycan 4 (PRG4) production.
  • Collagen fibrils in the superficial layer exhibited alignment similar to native cartilage surface zones.

Conclusions:

  • Mimicking synovial fluid flow in bioreactors is a promising strategy for engineering cartilage with a functional superficial zone.
  • This approach could be key to developing engineered cartilage that integrates effectively and mimics native tissue's zonal architecture.