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[Migration of PKH26-labeled mesenchymal stem cells in rats with Alzheimer's disease]
Wen-yu Li1, Ri-long Jin, Xing-yue Hu
1Psychology Department, Zhejiang University, Hangzhou, China.
Objective:
To investigate the migration of fluorescent dye PKH26-labeled BM-MSC in the Alzheimer's model rats.
Methods:
Normal human bone marrow extracted for isolation of BM-MSC was cultured in vitro. The 5th passaged BM-MSC was labeled with PKH26, and observed under a fluorescence microscope for PKH26 labeling efficiency, and using flow cytometry BM-MSC surface markers was checked. The PKH26 labeled BM-MSC injected into the tail vein of the normal control group and AD animal model group, 14 days after finding the PKH26-labeled BM-MSC cells in the rat hippocampus using fluorescence microscopy. Using the Morris water maze experiment comparison of AD model and BM-MSC transplantation group of spatial learning and memory ability.
Results:
TFlow cytometry showed BM-MSC surface markers CD73 and CD105 were positive. In vitro, PKH26-labeled rate of BM-MSC was 100 %. The Morris water maze experiment comparison of BM-MSC transplantation group and AD group of animals, BM-MSC transplantation group at 13, 14 days of spatial learning and memory ability than AD animal group had significantly improved. 14 days after BM-MSCs in rat hippocampus could be found which were PKH26-positive, consistent with DAPI staining. PKH26-positive cells in animal models of AD were significantly more than those in the normal control group.
Conclusion:
BM-MSC in AD rats not only migrates through the blood-brain barrier, but also mainly survives in the hippocampus of AD rats, and it can improve AD rat model of learning disabilities.
Insights
Bone marrow-derived mesenchymal stem cells (BM-MSC) migrate to the hippocampus in Alzheimer
Area of Science:
- Neuroscience
- Regenerative Medicine
- Cell Biology
Background:
- Alzheimer's disease (AD) is a progressive neurodegenerative disorder characterized by cognitive decline.
- Investigating therapeutic strategies for AD, including cell-based therapies, is crucial.
- Bone marrow-derived mesenchymal stem cells (BM-MSC) show potential for neural repair.
Purpose of the Study:
- To investigate the migration and survival of fluorescent dye PKH26-labeled BM-MSC in an Alzheimer's disease rat model.
- To evaluate the therapeutic effect of BM-MSC transplantation on cognitive function in AD rats.
Main Methods:
- BM-MSC were isolated, cultured, and labeled with PKH26.
- PKH26-labeled BM-MSC were intravenously injected into AD model rats and control rats.
- Cell migration to the hippocampus was assessed using fluorescence microscopy.
- Spatial learning and memory were evaluated using the Morris water maze test.
Main Results:
- PKH26-labeled BM-MSC successfully migrated to the hippocampus of AD rats within 14 days.
- BM-MSC transplantation significantly improved spatial learning and memory in AD rats compared to the control AD group.
- A higher number of PKH26-positive cells were observed in the hippocampus of AD rat models compared to normal controls.
Conclusions:
- BM-MSC can cross the blood-brain barrier and primarily survive in the hippocampus of AD rats.
- Transplanted BM-MSC demonstrate a therapeutic effect, improving learning disabilities in an Alzheimer's disease rat model.
- This study supports the potential of BM-MSC as a cell therapy for Alzheimer's disease.
