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Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper (cTfh) Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry
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CD4+ CD8+ T cell reference values in the Mexico City population.

Mónica Moreno-Galván1, Aracely Palafox

  • 1Hospital Infantil de México Federico Gómez, SSA, Mexico City, Mexico.

Clinical and Vaccine Immunology : CVI
|December 15, 2012
PubMed
Summary

This study establishes crucial CD4(+) and CD8(+) T cell reference values for healthy Mexicans. These findings provide essential flow cytometry standards for the Mexican population, aiding in accurate health assessments.

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Area of Science:

  • Immunology
  • Flow Cytometry
  • Clinical Reference Standards

Background:

  • Accurate lymphocyte subpopulation data is vital for clinical diagnostics.
  • Establishing population-specific reference values is necessary for reliable flow cytometry interpretation.
  • Previous data for healthy Mexicans is limited, necessitating this study.

Purpose of the Study:

  • To determine reference values for CD4(+) and CD8(+) T cells in healthy Mexicans.
  • To analyze these values based on gender and age.
  • To provide essential flow cytometry standards for the Mexican population.

Main Methods:

  • Flow cytometry analysis of peripheral blood samples.
  • Stratification of T cell subset data by gender and age groups.
  • Statistical analysis to establish reference ranges.

Main Results:

  • Established reference ranges for CD4(+) and CD8(+) T cell counts in healthy Mexican adults.
  • Observed variations in T cell proportions across different age groups and genders.
  • Data provides a baseline for immunological assessments in Mexico.

Conclusions:

  • The study successfully generated gender- and age-specific reference values for CD4(+) and CD8(+) T cells in Mexicans.
  • These values serve as critical standards for flow cytometry in clinical and research settings.
  • This work supports improved diagnostic accuracy and health monitoring within the Mexican population.