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Biomolecular Interactions of small-molecule inhibitors affecting the YopH protein tyrosine phosphatase
Megan Hogan1, Medhanit Bahta, Scott Cherry
1United States Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702, USA.
Abstract:
We have developed competitive and direct binding methods to examine small-molecule inhibitors of protein tyrosine phosphatase activity. Focusing on the Yersinia pestis outer protein H, a potent bacterial protein tyrosine phosphatase, we describe how an understanding of the kinetic interactions involving Yersinia pestis outer protein H, peptide substrates, and small-molecule inhibitors of protein tyrosine phosphatase activity can be beneficial for inhibitor screening, and we further translate these results into a microarray assay for high-throughput screening.
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