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Quantification of IncP-1 plasmid prevalence in environmental samples
Sven Jechalke1, Simone Dealtry, Kornelia Smalla
1Julius Kühn-Institut-Federal Research Center for Cultivated Plants, Department of Epidemiology and Pathogen Diagnostics, Braunschweig, Germany.
Applied and Environmental Microbiology
|December 18, 2012
Abstract:
To study the role of broad-host-range IncP-1 plasmids in bacterial adaptability to irregular environmental challenges, a quantitative real-time PCR assay was developed that specifically detects the korB gene, which is conserved in all IncP-1 plasmids, in environmental samples. IncP-1 plasmid dynamics in a biopurification system for pesticide wastes were analyzed.
