Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 16, 2026

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells
12:52

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

Published on: November 28, 2015

PDGFRA gain in low-grade diffuse gliomas.

Kazuya Motomura1, Michel Mittelbronn, Werner Paulus

  • 1International Agency for Research on Cancer, Lyon, France.

Journal of Neuropathology and Experimental Neurology
|December 18, 2012
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Progression of Low-Grade Neuroendocrine Tumors (NET) to High-Grade Neoplasms Harboring the NEC-Like Co-alteration of RB1 and TP53.

Endocrine pathology·2024
Same author

Consensus recommendations for an integrated diagnostic approach to peripheral nerve sheath tumors arising in the setting of Neurofibromatosis Type 1.

Neuro-oncology·2024
Same author

High-Grade Progression, Sarcomatous Transformation, and/or Metastasis of Pituitary Neuroendocrine Neoplasms (PitNENs): The UCSF Experience.

Endocrine pathology·2024
Same author

Epithelioid angiosarcoma arising from pleomorphic xanthoastrocytoma, CNS WHO grade 3.

Journal of neuropathology and experimental neurology·2024
Same author

cIMPACT-NOW update 8: Clarifications on molecular risk parameters and recommendations for WHO grading of meningiomas.

Neuro-oncology·2024
Same author

Meningeal solitary fibrous tumor cell states phenocopy cerebral vascular development and homeostasis.

Neuro-oncology·2024

Platelet-derived growth factor receptor-α (PDGFRA) amplification is common in diffuse astrocytomas, often occurring with IDH1/2 mutations. This finding offers insights into glioma development and patient prognosis.

Area of Science:

  • Neuro-oncology
  • Cancer Genetics
  • Molecular Pathology

Background:

  • Glioblastomas with proneural signatures often have IDH1 mutations and PDGFRA amplification.
  • IDH1/2 mutations are early events in diffuse astrocytomas, precursors to secondary glioblastomas.
  • The role and timing of PDGFRA amplification in these precursor tumors remain unclear.

Purpose of the Study:

  • To investigate the frequency and significance of PDGFRA amplification in low-grade diffuse gliomas.
  • To explore the relationship between PDGFRA amplification, IDH1/2 mutations, and 1p/19q loss.
  • To assess the prognostic value of PDGFRA amplification in diffuse astrocytomas.

Main Methods:

  • Quantitative polymerase chain reaction (qPCR) was used to assess PDGFRA gain in 342 low-grade diffuse gliomas.

More Related Videos

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma
09:17

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma

Published on: September 13, 2022

Related Experiment Videos

Last Updated: May 16, 2026

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells
12:52

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

Published on: November 28, 2015

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma
09:17

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma

Published on: September 13, 2022

  • Previously published data were analyzed to correlate PDGFRA gain with IDH1/2 mutations and 1p/19q loss.
  • Dual-color fluorescence in situ hybridization (FISH) was employed to examine PDGFRA and MET co-amplification patterns.

    • Main Results:

    • PDGFRA gain was detected in 16.3% of diffuse astrocytomas, significantly more than in oligodendrogliomas (2.6%).
    • PDGFRA gain showed an inverse correlation with IDH1/2 mutations and 1p/19q loss.
    • Diffuse astrocytomas frequently exhibited either IDH1/2 mutations or PDGFRA gain (93%).
    • Mean survival for diffuse astrocytoma patients with PDGFRA gain was 8.8 years, comparable to IDH1/2 or TP53 mutations, but longer than MET gain.
    • FISH analysis revealed PDGFRA and MET amplification in distinct cell populations, indicating intratumoral heterogeneity.

    Conclusions:

    • PDGFRA amplification is a frequent genetic event in diffuse astrocytomas, distinct from oligodendrogliomas.
    • The findings suggest PDGFRA amplification plays a role in diffuse astrocytoma development, potentially alongside IDH1/2 mutations.
    • PDGFRA amplification is associated with a favorable prognosis in diffuse astrocytomas.
    • Intratumoral heterogeneity, with distinct PDGFRA and MET amplification patterns, exists even in low-grade gliomas.