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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Related Experiment Video

Updated: May 16, 2026

Gene Expression Analyses in Human Follicles
09:09

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Published on: February 17, 2023

Microarray analysis identifies COMP as the most differentially regulated transcript throughout in vitro follicle

Robin M Skory1, Beatriz Peñalver Bernabé, Eugene Galdones

  • 1Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA.

Molecular Reproduction and Development
|December 18, 2012
PubMed
Summary
This summary is machine-generated.

Cartilage oligomeric matrix protein (COMP) is upregulated in cultured follicles, indicating its potential as a marker for follicle maturation. This finding aids in fertility preservation research for cancer patients.

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Published on: June 16, 2021

Area of Science:

  • Reproductive Biology
  • Molecular Endocrinology
  • Cell Biology

Background:

  • In vitro follicle growth is a key technology for studying folliculogenesis.
  • It offers potential fertility preservation methods for cancer patients.
  • The transcriptome and secretome of in vitro-grown follicles are not fully characterized.

Purpose of the Study:

  • To characterize the transcriptome and secretome of in vitro-grown follicles using microarray analysis.
  • To identify novel genes and proteins involved in follicle maturation.
  • To evaluate the potential of identified gene products as maturation markers.

Main Methods:

  • Microarray analysis was performed on in vitro-grown follicles.
  • Quantification of gene and protein expression, including Cartilage Oligomeric Matrix Protein (COMP).
  • Immunohistochemical localization of COMP in cultured and in vivo follicles.

Main Results:

  • Cartilage oligomeric matrix protein (COMP) mRNA was significantly upregulated in late-stage cultured follicles.
  • COMP protein was detected in the culture medium and localized to mural granulosa cells.
  • COMP expression correlated with known maturation markers (inhibin β(A), gremlin) and was absent in apoptotic follicles.

Conclusions:

  • COMP is a prominent gene product regulated during in vitro follicle maturation.
  • COMP shows potential as a reliable marker for assessing follicle maturation.
  • Further investigation into COMP's role could advance fertility preservation technologies.