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Single molecule fluorescent in situ hybridization (smFISH) of C. elegans worms and embryos

Ni Ji1, Alexander van Oudenaarden

  • 1Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Wormbook : the Online Review of C. Elegans Biology
|December 18, 2012
PubMed
Abstract

No abstract available in PubMed .

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...