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Intracranial Subarachnoidal Route of Infection for Investigating Roles of Streptococcus suis Biofilms in Meningitis in a Mouse Infection Model
Published on: July 1, 2018
Tonsilliphilus suis gen. nov., sp. nov., causing tonsil infections in pigs
Ryozo Azuma1, Bak Ung-Bok2, Satoshi Murakami3
12-7-33 Higashi-tokura, Kokubunji-city, Tokyo 185-0002, Japan.
International Journal of Systematic and Evolutionary Microbiology
|December 18, 2012
Summary
This study introduces Tonsilliphilus suis gen. nov., sp. nov., a novel bacterial species identified from pig lymph nodes and tonsils. This aerotolerant anaerobe exhibits unique cellular morphologies and distinct biochemical characteristics.
Area of Science:
- Microbiology
- Bacteriology
- Taxonomy
Background:
- A group of 17 bacterial strains, including strain W254(T), were isolated from swine submandibular lymph nodes and tonsils.
- These strains exhibited characteristics suggesting a potential novel genus and species within the Dermatophilaceae family.
Purpose of the Study:
- To taxonomically classify and characterize 17 bacterial isolates from pigs.
- To determine the phylogenetic placement of these isolates within the bacterial domain.
Main Methods:
- Polyphasic taxonomic analysis, including cellular morphology, biochemical tests, and chemotaxonomic analyses (peptidoglycan, whole-cell sugars, phospholipids, fatty acids, menaquinone).
- 16S rRNA gene sequence analysis for phylogenetic assessment.
- Isolation and characterization under specific atmospheric conditions (100% CO2).
Main Results:
- The 17 strains were identified as aerotolerant anaerobes with complex cellular morphologies, including thallus, tuber-like cells, coccoid forms, and zoospores.
- Biochemical tests revealed nitrate reduction positivity and negativity for catalase, indole, urea hydrolysis, and gelatin liquefaction.
- Chemotaxonomic data (meso-diaminopimelic acid, specific sugars, phospholipids, fatty acids, MK-8 (H4)) and 16S rRNA gene sequencing supported the establishment of a new genus and species.
Conclusions:
- The 17 strains represent a novel species within a new genus, proposed as Tonsilliphilus suis gen. nov., sp. nov.
- The type strain W254(T) (ATCC 35846(T)) is designated for this new taxon.
- This research expands the understanding of bacterial diversity in swine-associated environments.
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