Dietary guanidino acetic acid is an efficacious replacement for arginine for young chicks

Guanidino acetic acid (GAA) is synthesized in the liver and kidney from Arg and Gly and subsequently methylated by S-adenosylmethionine to form creatine. Four bioassays were carried out to determine the capacity of GAA to serve as a dietary replacement for Arg for growing chicks. Broiler chicks were fed Arg-deficient dextrose-casein (0.88% Arg) or corn-corn coproduct-soybean meal (1.0% Arg) basal diets during 9-d battery feeding trials involving 5 pens of 4 chicks per treatment. The dextrose-casein diet was shown to be markedly deficient in Arg as both weight gain and G:F increased (P < 0.01) due to addition of Arg, GAA, or creatine. The optimal level of added GAA was 0.12% of the diet, but this level of GAA or 1.0% creatine-H(2)O did not improve growth performance when added to an Arg-adequate diet. A second assay confirmed this level of optimal Arg in a 2 × 2 factorial arrangement of l-Arg and GAA supplementation. Using a practical-type diet based on corn, corn gluten meal, distillers dried grains with solubles, and soybean meal, similar improvements (P < 0.05) in G:F resulted from addition of 0.25% Arg, 0.12% GAA, or 0.15% creatine·H(2)O. These results demonstrate that 0.12% supplemental GAA, like creatine, produces consistent growth responses in young chicks fed Arg-deficient diets. To provide further evidence of the capacity for GAA to serve as a dietary Arg replacement, the dextrose-casein diet was supplemented with 7 graded doses of Arg in the absence or presence of 0.12% GAA (14 total diets). Quadratic (P < 0.01) responses in weight gain and G:F responses to supplemental Arg were observed. Similar supplemental Arg requirements were estimated in the absence and presence of 0.12% GAA, but GAA elicited a greater improvement (P < 0.05) in G:F when added to Arg-deficient, compared with Arg-adequate, diets. Collectively, these data indicate that GAA can be used as an efficacious replacement for dietary Arg for young chicks.