Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 16, 2026

Fabrication and Characterization of Disordered Polymer Optical Fibers for Transverse Anderson Localization of Light
09:19

Fabrication and Characterization of Disordered Polymer Optical Fibers for Transverse Anderson Localization of Light

Published on: July 29, 2013

Enzyme activity assays within microstructured optical fibers enabled by automated alignment.

Stephen C Warren-Smith1, Guiying Nie, Erik P Schartner

  • 1Institute for Photonics & Advanced Sensing (IPAS) and School of Chemistry & Physics, The University of Adelaide, South Australia 5005, Australia.

Biomedical Optics Express
|December 18, 2012
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dynamic multimode fiber specklegram sensor with automated training data generation for bed-exit prediction.

Biomedical optics express·2026
Same author

Embryo Implantation: New Molecular Insights in Endometrial Receptivity, Trophoblast Invasion and Signalling-An Introduction.

Biomolecules·2026
Same author

Podocalyxin may be down-regulated by progesterone in high grade serous ovarian cancer spheroids to increase responsiveness to carboplatin and NK cells.

BMC cancer·2026
Same author

The Role of Iron in Adipose Tissue Function, Crosstalk, and Transdifferentiation.

Cell biochemistry and function·2026
Same author

In-Line Tapered Microfiber Sensors for Label-Free Simultaneous Detection of Dual Genes via Enzymatic Recombinase Amplification.

ACS sensors·2026
Same author

A rigorous theoretical model of fluorescence-based fiber optic sensors: application to D-shaped fibers.

Scientific reports·2025

Researchers developed a novel fluorescence enzyme assay using microstructured optical fibers (MOF). This system, stabilized by automated alignment, enables sensitive detection of enzymes like proprotein convertase 5/6 (PC6), crucial for women

Area of Science:

  • Biomedical Engineering
  • Optical Sensing
  • Enzyme Assays

Background:

  • Enzyme activity assays are critical for diagnostics and research.
  • Microstructured optical fibers (MOFs) offer unique platforms for sensing applications.
  • Proprotein convertase 5/6 (PC6) is an enzyme with significant implications in women's health.

Purpose of the Study:

  • To demonstrate a fluorescence-based enzyme activity assay within a small-core microstructured optical fiber (MOF).
  • To develop and implement a reflection-based automated alignment system for stable optical measurements.
  • To validate the assay using the proprotein convertase 5/6 (PC6) enzyme.

Main Methods:

  • Development of a reflection-based automated alignment system utilizing feedback and piezoelectric actuators.
Keywords:
(060.2370) Fiber optics sensors(060.4005) Microstructured fibers(280.1415) Biological sensing and sensors

More Related Videos

Label-free Single Molecule Detection Using Microtoroid Optical Resonators
08:53

Label-free Single Molecule Detection Using Microtoroid Optical Resonators

Published on: December 29, 2015

Fabrication and Testing of Microfluidic Optomechanical Oscillators
09:10

Fabrication and Testing of Microfluidic Optomechanical Oscillators

Published on: May 29, 2014

Related Experiment Videos

Last Updated: May 16, 2026

Fabrication and Characterization of Disordered Polymer Optical Fibers for Transverse Anderson Localization of Light
09:19

Fabrication and Characterization of Disordered Polymer Optical Fibers for Transverse Anderson Localization of Light

Published on: July 29, 2013

Label-free Single Molecule Detection Using Microtoroid Optical Resonators
08:53

Label-free Single Molecule Detection Using Microtoroid Optical Resonators

Published on: December 29, 2015

Fabrication and Testing of Microfluidic Optomechanical Oscillators
09:10

Fabrication and Testing of Microfluidic Optomechanical Oscillators

Published on: May 29, 2014

  • Integration of the alignment system with a small-core microstructured optical fiber (MOF) for fluorescence detection.
  • Performance of a fluorescence-based enzyme activity assay targeting proprotein convertase 5/6 (PC6).

    • Main Results:

    • Successful demonstration of a fluorescence-based enzyme activity assay within a MOF.
    • The automated alignment system maintained optical stability throughout the assay duration.
    • The assay showed sensitivity for detecting proprotein convertase 5/6 (PC6) activity.

    Conclusions:

    • A novel MOF-based fluorescence enzyme assay has been successfully developed.
    • Automated optical alignment is crucial for stable and reliable enzyme activity measurements in MOFs.
    • This technology holds promise for applications in women's health diagnostics.