Related Experiment Video
Updated: May 16, 2026
08:26
Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
Published on: November 23, 2021
The chemistry of small-molecule fluorogenic probes
Jonathan B Grimm1, Laurel M Heckman, Luke D Lavis
1Janelia Farm Research Campus, The Howard Hughes Medical Institute, Ashburn, Virginia, USA.
Progress in Molecular Biology and Translational Science
|December 19, 2012
Summary
Chemical fluorophores are essential tools in biology for detection and visualization. This study explores fluorogenic molecules, where fluorescence is activated by chemical or biochemical triggers, enabling advanced bioassays and imaging.
Area of Science:
- Chemical Biology
- Biotechnology
- Molecular Imaging
Background:
- Chemical fluorophores are widely utilized in biological research for detection and visualization.
- Small-molecule dyes offer a significant advantage due to their constructability for fluorescence activation via chemical or biochemical processes.
Purpose of the Study:
- To detail a collection of fluorophores.
- To highlight strategies for controlling fluorescence using chemistry.
- To showcase unique approaches in fluorophore design.
Main Methods:
- Review and compilation of existing fluorophore designs.
- Analysis of chemical and biochemical activation mechanisms.
- Categorization of fluorescence control strategies.
Main Results:
- Identification of diverse fluorophore structures and their activation triggers.
- Demonstration of chemical and biochemical processes controlling fluorescence.
- Presentation of general and unique strategies for fluorescence modulation.
Conclusions:
- Fluorogenic molecules activated by enzymatic activity, light, or environmental changes are key for advanced bioassays.
- Chemistry-driven control over fluorescence enables sophisticated biological imaging experiments.
- The presented collection and strategies offer valuable insights for developing novel fluorescent probes.
More Related Videos
Related Concept Videos
03:31Labeling DNA Probes
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
01:14Photoluminescence: Applications
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
01:19Protein Dynamics in Living Cells
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
01:26Variables Affecting Phosphorescence and Fluorescence
Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...