Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Neurogenesis and Regeneration of Nervous Tissue01:15

Neurogenesis and Regeneration of Nervous Tissue

In the CNS, neurogenesis, the birth of new neurons from stem cells, is limited to the hippocampus in adults. In other regions of the brain and spinal cord, neurogenesis is almost non-existent due to inhibitory influences from neuroglia, especially oligodendrocytes, and the absence of growth-stimulating cues. The myelin produced by oligodendrocytes in the CNS inhibits neuronal regeneration. Furthermore, astrocytes proliferate rapidly after neuronal damage, forming scar tissue that physically...
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Pleiotropy01:33

Pleiotropy

Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Overlapping expression and co-operative function of the zebrafish pcdh15 paralogs.

Communications biology·2026
Same author

CFAP20 salvages arrested RNAPII from the path of co-directional replisomes.

Nature·2026
Same author

The homeobox gene, dmbx1a, is required for the development and maintenance of bipolar and photoreceptor cells in the zebrafish retina.

Experimental eye research·2025
Same author

Novel regulators of heparan sulfate proteoglycans modulate cellular uptake of α-synuclein fibrils.

Communications biology·2025
Same author

Broadly reactive anti-VHH antibodies for characterizing, blocking, or activating nanobody-based CAR-T cells.

Antibody therapeutics·2025
Same author

Students Perceive Similar Gains in Collaboration, Communication and Professional Skills in Two Distinct Experiential Learning Courses.

Pharmacology research & perspectives·2025

Related Experiment Video

Updated: May 15, 2026

Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells (NPCs)
10:47

Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells (NPCs)

Published on: March 2, 2018

Cockayne syndrome b maintains neural precursor function.

Raffaele Sacco1, Laura Tamblyn, Nishani Rajakulendran

  • 1Department of Pharmacology and Toxicology, University of Toronto, Toronto, Ontario, Canada.

DNA Repair
|December 19, 2012
PubMed
Summary

Cockayne Syndrome B (CSB) protein is crucial for neural stem cell self-renewal and neurite outgrowth, particularly after DNA damage. This study reveals CSB's vital role in neural precursor function and development.

More Related Videos

Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue
09:17

Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue

Published on: March 4, 2015

Related Experiment Videos

Last Updated: May 15, 2026

Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells (NPCs)
10:47

Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells (NPCs)

Published on: March 2, 2018

Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue
09:17

Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue

Published on: March 4, 2015

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Cockayne syndrome (CS) is a hereditary disorder linked to neurodevelopmental defects.
  • Cockayne Syndrome B (CSB) protein is involved in DNA repair, chromatin remodeling, and mitochondrial function.
  • The specific role of CSB in neural development remains unclear.

Purpose of the Study:

  • To investigate the function of Cockayne Syndrome B (CSB) in neural precursor cells.
  • To determine CSB's role in neural stem cell self-renewal and response to DNA damage.

Main Methods:

  • Analysis of neural progenitor abundance and apoptosis in Csb(-/-) mice.
  • In vitro neurosphere assays to assess neural precursor self-renewal.
  • In vitro UV irradiation experiments on neural precursors and differentiated neurons.

Main Results:

  • Csb(-/-) neural precursors show defective self-renewal in vitro, which worsens with serial passaging.
  • CSB and XPA preserve embryonic neural stem cell self-renewal after UV DNA damage.
  • UV-irradiated Csb(-/-) neurons exhibit impaired neurite outgrowth, despite normal lineage commitment.

Conclusions:

  • CSB plays a significant role in maintaining neural precursor self-renewal.
  • CSB is essential for the proper response of neural precursors to DNA damage, impacting neurite outgrowth.
  • These findings highlight a novel function for CSB in neural development.