Specific DNA detection using antibody mediated fluorescence quenching
Frank Sellrie1, Elmara Graser, Christine Lenz
1UP Transfer GmbH, Am Neuen Palais 10, 14469 Potsdam, Germany. frank.sellrie@up-transfer.de
Biosensors & Bioelectronics
|December 19, 2012
Abstract:
First homogenous immunoassay for sequence-specific nucleic acid detection is developed. The assay bases on our finding that a fluorophore inserted into a DNA probe instead of one of the internal nucleotides may get protected from fluorescence quenching caused by an anti-fluorophore antibody, if the probe is hybridized with the target sequence. This ensures a positive signal in the antibody presence. The assay enables quantitative detection and may have potential for development of homogenous high-throughput platforms.
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