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Related Experiment Video

Updated: May 15, 2026

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

Genetic variations in miR-27a gene decrease mature miR-27a level and reduce gastric cancer susceptibility.

Q Yang¹, Z Jie², S Ye³

¹Institute for Chemical Carcinogenesis, State Key Laboratory of Respiratory Disease, Guangzhou Medical University, Guangzhou, People's Republic of China.

|December 19, 2012

Summary

This summary is machine-generated.

Single-nucleotide polymorphisms (SNPs) in microRNA (miRNA) genes, like miR-27a, can alter gastric cancer risk. The rs11671784 G/A polymorphism in miR-27a decreases its expression, reducing gastric cancer susceptibility.

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miRNA Expression Analyses in Prostate Cancer Clinical Tissues

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

Area of Science:

Genetics
Molecular Biology
Oncology

Background:

MicroRNAs (miRNAs) are key post-transcriptional regulators of oncogenes and tumor suppressors.
Single-nucleotide polymorphisms (SNPs) in miRNA genes represent a novel class of genetic variations investigated in cancer.
Gastric cancer susceptibility is influenced by various genetic and environmental factors.

Purpose of the Study:

To investigate the impact of SNPs in the miR-27a gene on miR-27a expression.
To determine the association between miR-27a gene polymorphisms and gastric cancer risk.
To elucidate the functional consequences of miR-27a gene variations in gastric tumorigenesis.

Main Methods:

A case-control population study was conducted to examine allele and genotype frequencies of the rs11671784 polymorphism in the miR-27a gene.
In vitro studies involved overexpressing pre-miR-27a variants to confirm functional effects.
Expression levels of mature miR-27a and its target HOXA10 were analyzed.

Main Results:

The G/A polymorphism in the miR-27a gene (rs11671784) significantly affected gastric cancer risk.
GA heterozygotes and AA homozygotes exhibited a decreased risk of gastric cancer compared to GG homozygotes.
The G/A polymorphism impaired pre-miR-27a processing, reducing mature miR-27a expression and increasing HOXA10 levels.

Conclusions:

The G/A polymorphism in miR-27a (rs11671784) is associated with decreased miR-27a expression and reduced gastric cancer risk.
This polymorphism plays a role in gastric tumorigenesis by affecting miRNA processing and target gene regulation.
Findings provide insights into miRNA SNPs' contribution to cancer pathogenesis and individual susceptibility assessment.