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Updated: May 15, 2026

Co-localization of Cell Lineage Markers and the Tomato Signal
Published on: December 28, 2016
Overlapping glycosylation sequon influences the glycosylation pattern of a chimeric protein expressed in tomato leaf
Marni E Cueno1, Kenichi Imai, Takashi Okamoto
1Department of Molecular and Cellular Biology, Nagoya City University, Aichi 467-8601, Japan. marni.cueno@nihon-u.ac.jp
Abstract:
Overlapping glycosylation sequon (OGS) is composed of two overlapping N-glycosylation sequons and has been found in certain glycoproteins with pharmaceutical value. With a growing interest to produce pharmaceutical glycoproteins in plants, it is important to establish the glycosylation pattern of OGS-containing proteins expressed in varying plant tissues. Here, a chimeric OGS (NNST)-containing gene and its mutated forms (NNAT and NASNAT) were expressed in callus-derived tomato plantlets. Tissue extracts from the recombinant leaf and callus were used in immunoblotting and glycoprotein detection. We found that the glycosylation patterns of the NNST-containing chimeric protein differ from that of NNAT- and NASNAT-containing proteins.
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