Related Experiment Video
Updated: May 15, 2026

Removal of Exogenous Materials from the Outer Portion of Frozen Cores to Investigate the Ancient Biological Communities Harbored Inside
Published on: July 3, 2016
Characterization of Thauera-dominated hydrogen-oxidizing autotrophic denitrifying microbial communities by using
Yanping Mao1, Yu Xia, Tong Zhang
1Environmental Biotechnology Laboratory, Department of Civil Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong.
Abstract:
The present study, for the first time, reported a Thauera-dominated hydrogen-oxidizing autotrophic denitrifying microbial community enriched from different seed sludges including activated sludge and anaerobic digestion sludge. After 244 days enrichment, nitrogen removal rates reached up to 0.2 mg N/mg VSS/d which were comparable to that of the model organism Paracoccus denitrificans under the same conditions. Furthermore, high-throughput sequencing was applied to characterize and compare the seed sludges and enriched cultures. Operational taxonomic units (OTU)-based analysis (97% similarity cutoff) of total 280,000 16S rRNA gene V6 region sequences from 7 sludge samples (40,000 sequences per sample) revealed that the microbial diversity decreased after the enrichment, indicated by OTU numbers drop of 55-60%. Thauera species in the class of β-Proteobacteria were enriched into the dominant populations with relative abundances of 47-62%, regardless of seed sludge sources.
More Related Videos
Related Concept Videos
Deep Sea Microbial Ecology
Hyperthermophilic Bacteria
Microbial Mats
Diversity of Archaea I
Diversity of Archaea II
Diversity of Archaea III

