Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Biofuels01:25

Biofuels

The microbial conversion of organic matter into biofuels holds potential as a renewable energy source. Among biofuel sources, microalgae are recognized as a highly efficient and adaptable feedstock for biodiesel production, owing to their rapid biomass accumulation, elevated lipid productivity, and capacity to proliferate in diverse aquatic systems, including freshwater, marine, and wastewater habitats. Unlike terrestrial crops, microalgae do not compete for land and can achieve significantly...
Lipid Catabolism01:25

Lipid Catabolism

Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...
Production of Pharmaceuticals01:30

Production of Pharmaceuticals

Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...
Lipid Digestion01:06

Lipid Digestion

Lipids are large molecules that are generally not water-soluble. Since most of the digestive enzymes in the human body are water-based, there are specific steps the body must take to break down lipids and make them available for use.
Biosynthesis of Lipids01:29

Biosynthesis of Lipids

Microbial membranes exhibit remarkable diversity in lipid composition, reflecting evolutionary adaptations to various environmental conditions. The three domains of life—Bacteria, Archaea, and Eukarya—synthesize membrane lipids through distinct biosynthetic pathways, leading to fundamental structural differences that impact membrane stability, function, and adaptability.Fatty Acid-Based Lipids in Bacteria and EukaryaBacteria and eukaryotes share a common fatty acid biosynthesis pathway, which...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Therapeutic targeting of COX-2 in head and neck cancer: Mechanistic and clinical perspectives.

Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences·2026
Same author

Precision pharmacology of CNS GPCRs: From biased signaling to translational therapeutics.

Progress in neuro-psychopharmacology & biological psychiatry·2026
Same author

Microbiota-derived metabolites as nutritional signals in insulin resistance and obesity.

Clinical nutrition ESPEN·2026
Same author

Cloning and Heterologous Expression of Laccase from Beauveria pseudobassiana PHF4 in E. coli and its Application in the Degradation of Catechol.

Current microbiology·2026
Same author

[HEARTS quality: a policy framework to strengthen hypertension and cardiovascular risk management in primary healthcare - insights from HEARTS in the AmericasQualidade da iniciativa HEARTS: marco de políticas para fortalecer o manejo da hipertensão e do risco cardiovascular na atenção primária à saúde. Perspectivas da iniciativa HEARTS nas Américas].

Revista panamericana de salud publica = Pan American journal of public health·2026
Same author

Visual Recovery with Cryopreserved Amniotic Membrane After Epi-Off Corneal Cross-Linking in Keratoconus Patients: A Non-Randomized, Controlled, Retrospective Study.

Clinical ophthalmology (Auckland, N.Z.)·2026

Related Experiment Video

Updated: May 15, 2026

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass
09:10

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass

Published on: June 24, 2016

Biodiesel production by transesterification using immobilized lipase.

Sunil Kumar Narwal1, Reena Gupta

  • 1Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla 171005, India.

Biotechnology Letters
|December 19, 2012
PubMed
Summary

Enzymatic biodiesel production offers high purity and environmental benefits. Immobilizing lipases and optimizing reaction parameters can overcome cost and speed challenges, making it a viable alternative fuel source.

More Related Videos

Ultrasonic-Assisted Preparation of Biodiesel Products from Vegetable Oils
04:40

Ultrasonic-Assisted Preparation of Biodiesel Products from Vegetable Oils

Published on: April 19, 2024

Laboratory Production of Biofuels and Biochemicals from a Rapeseed Oil through Catalytic Cracking Conversion
11:33

Laboratory Production of Biofuels and Biochemicals from a Rapeseed Oil through Catalytic Cracking Conversion

Published on: September 2, 2016

Related Experiment Videos

Last Updated: May 15, 2026

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass
09:10

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass

Published on: June 24, 2016

Ultrasonic-Assisted Preparation of Biodiesel Products from Vegetable Oils
04:40

Ultrasonic-Assisted Preparation of Biodiesel Products from Vegetable Oils

Published on: April 19, 2024

Laboratory Production of Biofuels and Biochemicals from a Rapeseed Oil through Catalytic Cracking Conversion
11:33

Laboratory Production of Biofuels and Biochemicals from a Rapeseed Oil through Catalytic Cracking Conversion

Published on: September 2, 2016

Area of Science:

  • Biotechnology
  • Renewable Energy

Background:

  • Biodiesel is a renewable alternative fuel derived from vegetable or waste oils.
  • Transesterification, catalyzed by lipases, is a key process in biodiesel production.
  • Enzymatic routes offer higher purity compared to traditional chemical methods.

Purpose of the Study:

  • To explore the enzymatic production of biodiesel.
  • To identify challenges and solutions for lipase-catalyzed biodiesel synthesis.
  • To highlight key factors influencing biodiesel production efficiency.

Main Methods:

  • Transesterification of oils using lipases.
  • Enzyme modification to enhance stability and activity.
  • Enzyme immobilization on suitable supports for reusability.

Main Results:

  • Enzymatic biodiesel production yields high-purity products.
  • Enzyme immobilization addresses cost and reusability issues.
  • Optimization of reaction parameters is crucial for economic viability.

Conclusions:

  • Enzymatic biodiesel production is a promising green technology.
  • Overcoming lipase cost and reaction rate limitations is key to widespread adoption.
  • Further research into parameter optimization will enhance biodiesel production efficiency.