Related Experiment Video
Updated: May 15, 2026

Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS
Published on: November 8, 2015
Determination of azathioprine in bulk and pharmaceutical dosage form by HPTLC
Pritam S Jain1, Pradip Thakre, Amar J Chaudhari
1Department of Pharmaceutical Chemistry, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dist. Dhule, Maharashtra, India.
Introduction:
An HPTLC method for analysis of Azathioprine in bulk and pharmaceutical formulation has been established and validated.
Materials And Methods:
The analyte was separated on aluminium plates precoated with silica gel 60 F254. The mobile phase was Ethyl acetate: Methanol: Triethylamine (4:1:0.5v/v). Quantification was done by densitometric scanning at 300nm.
Results:
Response was a linear function of Azathioprine concentration in the range of 200-1200 ng/band. The limit of detection and quantification for Azathioprine were 18.58 and 59.14 ng/band, respectively. Average recovery was 100.1% which shows that the method was free from interference from excipients present in the formulation.
Conclusion:
The established method enabled accurate, precise, and rapid analysis of Azathioprine in bulk as well as pharmaceutical formulation.
More Related Videos
Related Concept Videos
Determination of Multiple Dosing Parameters: Loading and Maintenance Doses
Determination of Multiple Dosing Parameters: Steady-State, Minimum and Maximum Concentrations

