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Updated: May 15, 2026

Evaluation of Fluid Overload by Bioelectrical Impedance Vectorial Analysis
Published on: August 17, 2022
Is bioelectrical impedance spectroscopy accurate in estimating total body water and its compartments in elite
Catarina N Matias1, Diana A Santos, Ezequiel M Gonçalves
1Exercise and Health Laboratory, CIPER, Fac Motricidade Humana, Univ Tecn Lisboa, 1499-002 Cruz-Quebrada, Portugal.
Background:
Bioelectrical impedance spectroscopy (BIS) provides an affordable assessment of the body's various water compartments: total body water (TBW), extracellular water (ECW) and intracellular water (ICW). However, little is known of its validity in athletes.
Aim:
To validate TBW, ECW and ICW by BIS in elite male and female Portuguese athletes using dilution techniques (i.e. deuterium and bromide dilution) as criterion methods.
Subjects And Methods:
Sixty-two athletes (18.5 ± 4.1 years) had TBW, ECW and ICW assessed by BIS during their respective pre-season.
Results:
BIS significantly under-estimated TBW by 1.0 ± 1.7 kg and ICW by 0.9 ± 1.9 kg in relation to the criterion methods, with no differences observed for ECW. The values for the concordance correlation coefficient were 0.98 for TBW and ECW and 0.95 for ICW. Bland-Altman analyses revealed no bias for the various water compartments, with the 95% confidence intervals ranging from - 4.8 to 2.6 kg for TBW, - 1.5 to 1.6 kg for ECW and - 4.5 to 2.7 kg for ICW.
Conclusions:
Overall, these findings demonstrate the validity of BIS as a valid tool in the assessment of TBW and its compartments in both male and female athletes.
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