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Updated: May 15, 2026

A Protocol for Immunohistochemistry and RNA In-situ Distribution within Early Drosophila Embryo
Published on: May 6, 2022
BCAS2 is essential for Drosophila viability and functions in pre-mRNA splicing
Po-Han Chen1, Chia-I Lee, Yu-Tzu Weng
1Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei 100, Taiwan.
Abstract:
Here, we show that dBCAS2 (CG4980, human Breast Carcinoma Amplified Sequence 2 ortholog) is essential for the viability of Drosophila melanogaster. We find that ubiquitous or tissue-specific depletion of dBCAS2 leads to larval lethality, wing deformities, impaired splicing, and apoptosis. More importantly, overexpression of hBCAS2 rescues these defects. Furthermore, the C-terminal coiled-coil domain of hBCAS2 binds directly to CDC5L and recruits hPrp19/PLRG1 to form a core complex for splicing in mammalian cells and can partially restore wing damage induced by knocking down dBCAS2 in flies. In summary, Drosophila and human BCAS2 share a similar function in RNA splicing, which affects cell viability.
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