Related Experiment Video
Updated: May 15, 2026

Rapid Isolation of Wild Nematodes by Baermann Funnel
Published on: January 31, 2022
[Helminth prevalence in a waste-water plant at El Rosal, Cundinamarca]
Carolina Ortiz1, Myriam C López, Favio A Rivas
1Departamento de Salud Pública, Universidad Nacional de Colombia, Bogotá, Colombia. cortizpi@unal.edu.co
Objective:
Assessing helminth egg prevalence in sludge and raw and treated wastewater from a wastewater treatment system located in the village of El Rosal, Cundinamarca.
Methodology:
30 wastewater and 10 sludge samples from the El Rosal plant were taken during a 10-week period. The sludge and water samples were processed according to the Bailinger and the official Mexican standard methodology, respectively. Egg viability was determined by the method described by Victórica & Galván and the Mexican official standard.
Results:
Descriptive statistics were used for analysing data. 100 % of the untreated wastewater samples showed the presence of eggs and at least one viable helminth egg/litre was found in 90 % of them. 90 % of the treated wastewater samples were positive for the presence of eggs, finding that 70 % had at least one viable egg. All raw wastewater samples being dumped directly into the stream were positive for helminths; the same situation was found at the time of the viability test. All sludge samples were positive for helminths, finding that 100 % of these had at least one viable egg.
Conclusion:
Using this water for crop irrigation and using the sludge as fertiliser is a potential risk for public health. The sludge can only be used in forestry activities, as long as it does not come into contact with humans.
Related Concept Videos
Cholera
Anthelminthic Agents

