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Grape-seed proanthocyanidin extract as suppressors of bone destruction in inflammatory autoimmune arthritis
Jin-Sil Park1, Mi-Kyung Park, Hye-Joa Oh
1The Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, Republic of Korea.
Abstract:
Chronic autoimmune inflammation, which is commonly observed in rheumatoid arthritis (RA), disrupts the delicate balance between bone resorption and formation causing thedestruction of the bone and joints. We undertook this study to verify the effects of natural grape-seed proanthocyanidin extract (GSPE), an antioxidant, on chronic inflammation and bone destruction. GSPE administration ameliorated the arthritic symptoms of collagen-induced arthritis (CIA), which are representative of cartilage and bone destruction. GSPE treatment reduced the formation of tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells and osteoclast activity and increased differentiation of mature osteoblasts. Receptor activator of NFκB ligand expression in fibroblasts from RA patients was abrogated with GSPE treatment. GSPE blocked human peripheral blood mononuclear cell-derived osteoclastogenesis and acted as an antioxidant. GSPE improved the arthritic manifestations of CIA mice by simultaneously suppressing osteoclast differentiation and promoting osteoblast differentiation. Our results suggest that GSPE may be beneficial for the treatment of inflammation-associated bone destruction.
Insights
Grape seed proanthocyanidin extract (GSPE) shows promise in treating rheumatoid arthritis. This antioxidant suppressed inflammation and bone destruction by balancing osteoclast and osteoblast activity.
Area of Science:
- Biochemistry
- Immunology
- Rheumatology
Background:
- Chronic autoimmune inflammation, characteristic of rheumatoid arthritis (RA), leads to bone and joint destruction by disrupting bone remodeling.
- Understanding the molecular mechanisms underlying RA-associated bone loss is crucial for developing effective treatments.
Purpose of the Study:
- To investigate the effects of natural grape seed proanthocyanidin extract (GSPE) on chronic inflammation and bone destruction in the context of rheumatoid arthritis.
- To determine GSPE's impact on cellular processes involved in bone remodeling, specifically osteoclast and osteoblast differentiation.
Main Methods:
- Administration of GSPE to collagen-induced arthritis (CIA) mouse models.
- Assessment of GSPE's antioxidant properties.
- Evaluation of GSPE's effects on osteoclastogenesis and osteoblast differentiation in vitro using human peripheral blood mononuclear cells and RA patient fibroblasts.
- Measurement of tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells and receptor activator of NFκB ligand (RANKL) expression.
Main Results:
- GSPE administration ameliorated arthritic symptoms and reduced cartilage and bone destruction in CIA mice.
- GSPE treatment inhibited osteoclast differentiation and activity, evidenced by reduced TRAP-positive multinucleated cells.
- GSPE promoted osteoblast differentiation and suppressed RANKL expression in RA patient fibroblasts.
- GSPE demonstrated antioxidant activity, further contributing to its therapeutic effects.
Conclusions:
- GSPE effectively mitigates inflammation-associated bone destruction in a preclinical model of rheumatoid arthritis.
- GSPE exerts its beneficial effects by simultaneously inhibiting osteoclastogenesis and promoting osteoblastogenesis.
- GSPE holds potential as a therapeutic agent for managing rheumatoid arthritis and related bone pathologies.
