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Updated: May 15, 2026

Preparation of Hard Palm Seeds for Matrix-Assisted Laser Desorption/Ionization-Imaging Mass Spectrometry Analysis
Published on: June 30, 2023
[GC-MS analysis of chemical components in seeds oil from Croton tiglium]
Mei Lan1, Ping Wan, Zhi-Ying Wang
1Department of Gastroenterology, the Affiliated Hospital of Shaoxing University, Shaoxing 312000, China. meilsx@163.com
Objective:
To identify the chemical components and their relative content in seeds oil from Croton tiglium.
Methods:
The oil obtained by extracting of the seeds of Croton tiglium with petroleum ether was subjected to methyl-esterification or dilution with ethylether. GC-MS were used to identify the components in croton oil,peak area normalization method was used to determine the relative content of these substances in the sample.
Results:
Seventeen fat acid components were identified from croton oil. The main components were linoleic acid, oleic acid, and eicosenoic acid in methyl-esterified sample, whose quantities accouted for 77.33%. In addition, five aromatic compounds were also found in the sample diluted with ethylether, such as isoborneol, fenchyl alcohol, etc. Phorbol esters, having carcinogenesis and anti-HIV-1 effects, were not been identified.
Conclusion:
There are abundant of linoleic acid, oleic acid and eicosenoic acid in the seeds oil extracted from Chinese Croton tiglium. In contrast, the active component with carcinogenesis and anti-HIV-1 might be very rare in the samples and difficult to be obtained by ordinary separating and extracting methods.

