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Updated: May 15, 2026
Migratory Behavior of Cells Generated in Ganglionic Eminence Cultures
Published on: April 21, 2011
[Effect of polysaccharides from Radix Glycyrrhizae on migration and polyamines contents of IEC-6 cell]
Peng Wen1, Jing-Jing Sui, Ru-Liu Li
1Piwei Institute, Guangzhou University of Chinese Medicine, Guangzhou 510405, China. wenpengsweet@163.com
Objective:
To study the effect of polysaccharides from Radix Glycyrrhizae on migration and polyamines (putrescine, spermidine and spermine) contents of IEC-6 cell.
Methods:
Cell migration model was induced by scratch method in each well,and the polyamines in IEC-6 cell was determined by pre-column derivation high performance liquid chromatography. The polysaccharides inhibited effect on migration and polyamines contents of IEC-6 cells, and on IEC-6 cell migration by DFMO (a polyamines synthesis inhibitor) and the polyamines contents in the cells were observed.
Results:
The polysaccharides (50 mg/L or 100 mg/L) was able to promote the cell migration, reverse the cell migration inhibition by DFMO, enhance the IEC-6 cell polyamines (putrescine, spermidine and spermine) contents in the process of cell migration and reverse the reduction of polyamines (putrescine, spermidine and spermine) induced by DFMO.
Conclusion:
The effect of Radix Glycyrrhizae on the gastrointestinal mucosal damage repairing may be related to increasing polyamine content in cells and promoting cell migration.
