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Determination of percent hemoglobin A1c using a potentiometric method
1Department of Chemistry and Biochemistry, The University of Texas at Austin, 105 E. 24th Street, Stop A5300, Austin, Texas 78712-0165, United States.
Analytical Chemistry
|December 21, 2012
Summary
A new potentiometric method accurately measures hemoglobin A1c (HbA1c) and hemoglobin (Hb) in blood. This technique utilizes Alizarin red S and phenylboronic acid for precise diabetes mellitus monitoring.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Clinical Diagnostics
Background:
- Glycated hemoglobin (HbA1c) is crucial for diabetes mellitus diagnosis and management.
- Accurate measurement of HbA1c and total hemoglobin (Hb) is essential for clinical decision-making.
- Existing methods may require complex procedures or specialized equipment.
Purpose of the Study:
- To develop and validate a novel potentiometric method for simultaneous measurement of HbA1c and Hb concentrations.
- To establish a reliable and accessible method for determining percent HbA1c (%HbA1c) in human blood hemolysate.
- To provide an alternative diagnostic tool for diabetes management.
Main Methods:
- A potentiometric method employing Alizarin red S (ARS) as a redox indicator.
- Utilizing phenylboronic acid (PBA) for selective binding to HbA1c via diol-boronic acid complexation.
- Quantifying Hb concentration through its reaction with ferricyanide (Fe(CN)6(3-)).
Main Results:
- The method demonstrated a positive potential shift proportional to HbA1c concentration due to competitive binding with ARS for PBA.
- Hemoglobin concentration was determined by a potential shift proportional to the logarithm of Hb concentration.
- The %HbA1c results showed good agreement with a reference method, indicating high accuracy and reliability.
Conclusions:
- The developed potentiometric method offers a viable approach for measuring HbA1c and Hb concentrations.
- This technique provides accurate %HbA1c measurements, supporting diabetes mellitus diagnosis and management.
- The method's reliance on potentiometry suggests potential for cost-effective and accessible clinical application.
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