Related Experiment Video

Updated: May 15, 2026

Determination of the Glycogen Content in Cyanobacteria
07:04

Determination of the Glycogen Content in Cyanobacteria

Published on: July 17, 2017

Determination of percent hemoglobin A1c using a potentiometric method

Hong Liu1, Richard M Crooks

  • 1Department of Chemistry and Biochemistry, The University of Texas at Austin, 105 E. 24th Street, Stop A5300, Austin, Texas 78712-0165, United States.

Analytical Chemistry
|December 21, 2012
PubMed

We report a potentiometric method for measuring the hemoglobin A1c (HbA1c, glycated hemoglobin) concentration, hemoglobin (Hb) concentration, and percent HbA1c (%HbA1c) in human blood hemolysate. The %HbA1c is important for diagnosis and management of diabetes mellitus. Alizarin red s (ARS) is used as a redox indicator. Phenylboronic acid (PBA) binds to both ARS and HbA1c via diol-boronic acid complexation. The binding of PBA to ARS shifts its redox potential negatively. However, when HbA1c competes with ARS for PBA binding, the solution potential shifts positively. This shift is linked to the HbA1c concentration. The concentration of Hb is determined by allowing it to react with Fe(CN)(6)(3-). The potential shift arising from the reduction of Fe(CN)(6)(3-) by Hb is proportional to the logarithm of the Hb concentration. The results obtained for %HbA1c in human blood hemolysate are in good agreement with those determined using a reference method.

More Related Videos

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering05:18

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering

Published on: December 7, 2016

Analysis of Hematopoietic Stem Progenitor Cell Metabolism12:20

Analysis of Hematopoietic Stem Progenitor Cell Metabolism

Published on: November 9, 2019

Related Concept Videos

Potentiometry: Overview01:06

Potentiometry: Overview

Potentiometry is an analytical technique that measures the potential difference between two electrodes in an electrochemical cell without drawing any significant current that could alter the solution's composition. This method employs an indicator electrode, which exchanges electrons with the analyte solution, and a reference electrode with a constant potential. Each electrode is immersed in a solution comprised of two half-cells. In a conventional setup, the reference electrode serves as the...
Potentiometric Titration: Overview01:31

Potentiometric Titration: Overview

Potentiometric titration is a quantitative analytical technique that determines the concentration of an analyte by measuring the potential difference between the two electrodes in the solution. The endpoint of a potentiometric titration is the point at which there is a significant change in the potential difference. It occurs when the stoichiometric reaction between the analyte and the titrant is complete. The endpoint is usually determined graphically by plotting the measured potential...
Potentiometry: Types of Electrodes01:19

Potentiometry: Types of Electrodes

Reference electrodes serve as a stable reference point for potentiometric measurements, while indicator and working electrodes react to variations in the composition of a solution.
The Standard Hydrogen Electrode (SHE) is a widely used reference electrode that maintains zero potential across all temperatures. However, its need for a continuous hydrogen gas supply renders it impractical for everyday use.
An alternative to SHE is the Saturated Calomel Electrode (SCE). This electrode features an...
Controlled-Potential Coulometry: Electrolytic Methods01:17

Controlled-Potential Coulometry: Electrolytic Methods

Controlled-potential coulometry, also known as potentiostatic coulometry, employs a three-electrode system in which the working electrode's potential is precisely regulated using a potentiostat. Platinum working electrodes are utilized for positive potentials, while mercury pool electrodes are favored for extremely negative potentials. The platinum counter electrode is separated from the analyte using a membrane or salt bridge to avoid interference in the analysis.
The chosen potential ensures...
Amperometry: Overview01:10

Amperometry: Overview

Amperometry is a technique commonly used to measure the concentration of specific analytes in a solution by monitoring the electric current generated during an electrochemical reaction. It involves applying a constant potential between a working electrode and a reference electrode to measure the resulting current, which is proportional to the concentration of the analyte. The Clark oxygen electrode operates based on this principle of amperometry. It consists of a cathode and an anode enclosed...
Potentiometry: Membrane Electrodes01:15

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at the...