Related Experiment Video
Updated: May 15, 2026

One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode
Published on: June 18, 2016
Effects of Helicobacter pylori infection on MUC5AC protein expression in gastric cancer
Ding Shi1, Xin-min Qiu, Yin-feng Bao
1Department of Gastroenterology, The First People's Hospital of Yuhang District, Hangzhou 311100, Zhejiang Province, China. shidingyuhang@163.com
Aim:
To investigate the effects of the expression of the MUC5AC protein in gastric cancer depending on the Helicobacter pylori (Hp) infection status.
Materials & Methods:
The MUC5AC protein and mRNA were detected using western blot and real-time PCR protocols in gastric cancer tissue and stratified for Hp infection. Gastric mucus membranes near the cancer site serve as the control group.
Results:
The expression of MUC5AC protein and mRNA is significantly decreased in gastric cancer tissue (p < 0.05). The decrease was more significant in the Hp-infected group than in the Hp-uninfected group (p < 0.05).
Conclusion:
The infection of Hp is correlated with a decrease in MUC5AC protein amount in gastric cancer tissue. The current result suggests that there may be a potential necessary link between Hp, MUC5AC and gastric cancer.
Related Concept Videos
Gastritis II: Pathophysiology
Peptic Ulcer
Peptic Ulcer Disease II: Pathophysiology
Peptic Ulcer Disease II: Pathophysiology
Damaging agents such as Helicobacter pylori, gastric acid, pepsin, and nonsteroidal anti-inflammatory drugs (NSAIDs) can weaken the mucosal defense, allowing hydrogen ions to infiltrate back and harm epithelial cells.
Pathophysiology of Peptic Ulcer Disease: Injurious Factors
In the antrum region, G cells secrete the gastrin hormone that binds to gastrin-cholecystokinin-B (CCK2) receptors on parietal and enterochromaffin-like (ECL) cells in the fundic glands. Simultaneously, the vagus nerve releases acetylcholine, which binds to M3...
Pathophysiology of Peptic Ulcer Disease: Mucosal Defense Factors
