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A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

Published on: March 28, 2017

A novel comprehensive analysis method for Staphylococcus aureus pathogenicity islands.

Yusuke Sato'o¹, Katsuhiko Omoe, Hisaya K Ono

¹Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan.

Microbiology and Immunology

|December 21, 2012

Summary

Staphylococcus aureus pathogenicity islands (SaPIs) are mobile genetic elements that contribute to bacterial evolution. Researchers identified seven novel SaPIs using long and accurate PCR, revealing new insights into S. aureus pathogenicity.

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Area of Science:

Microbiology
Genetics
Molecular Biology

Background:

Staphylococcus aureus pathogenicity islands (SaPIs) are mobile genetic elements (MGEs) crucial for the evolution of S. aureus.
Many SaPIs harbor genes encoding staphylococcal enterotoxins and related toxins, influencing bacterial virulence.

Purpose of the Study:

To comprehensively investigate the diversity of SaPIs in S. aureus.
To identify novel SaPIs and understand their genetic makeup and distribution.

Main Methods:

Development and application of a long and accurate (LA)-PCR method targeting six SaPI insertion sites.
Restriction fragment length polymorphism (RFLP) and nucleotide sequencing for characterization of PCR products.

Main Results:

Identification of seven novel SaPIs (SaPIivm10, SaPishikawa11, SaPIivm60, SaPIno10, SaPIhirosaki4, SaPIj11, and SaPIhhms2).
These new SaPIs exhibit mosaic structures with known and unknown genetic components.
Significant variations in superantigen toxin production were observed among strains carrying different SaPIs.

Conclusions:

The LA-PCR approach is effective for comprehensively identifying SaPI diversity.
The discovered novel SaPIs contribute to the understanding of S. aureus evolution and pathogenicity.
SaPIs play a significant role in the variation of superantigen toxin production in S. aureus strains.