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Detection of IgG and IgE reactivity to BP180 using the ISAC® microarray system
D Pomponi1, G Di Zenzo, D Zennaro
1Center for Molecular Allergology, IDI-IRCCS, Via dei Monti di Creta 104, 00167 Rome, Italy.
The British Journal of Dermatology
|December 21, 2012
Summary
This study shows a microarray system can detect both IgE and IgG autoantibodies in bullous pemphigoid (BP) patients. This method is effective for diagnosing BP and understanding its link to allergies.
Area of Science:
- Immunology
- Dermatology
- Allergy Research
Background:
- Bullous pemphigoid (BP) is an autoimmune skin disease.
- Autoantibodies against BP180 and BP230 proteins are characteristic of BP.
- Both immunoglobulin G (IgG) and immunoglobulin E (IgE) play roles in BP pathogenesis.
Purpose of the Study:
- To assess the feasibility of using a microarray system for detecting IgE and IgG autoantibodies.
- To target the immunodominant NC16A domain of BP180.
- To compare microarray results with enzyme-linked immunosorbent assay (ELISA) findings.
Main Methods:
- BP180 antigen was immobilized on an experimental microarray (Exp96).
- Both BP patients and healthy donors were tested on commercial and experimental microarrays.
- IgG and IgE levels were measured simultaneously and compared to ELISA.
Main Results:
- The microarray system successfully replicated IgG ELISA results for BP patients.
- IgG and IgE autoantibodies against BP180 were detected in a significant proportion of BP patients.
- IgG levels correlated with disease severity, and IgE profiles showed associations with specific allergen sensitivities, notably hen's egg.
Conclusions:
- The ISAC microarray system is a viable tool for detecting IgG and IgE autoantibodies in bullous pemphigoid.
- This simultaneous detection method offers efficiency and potential for broader autoantigen screening.
- The system aids in understanding IgE-mediated allergic cross-reactivity in BP patients.
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