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Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
Published on: May 8, 2013
Correlation of cell membrane lipid profiles with daptomycin resistance in methicillin-resistant Staphylococcus aureus
Nagendra N Mishra1, Arnold S Bayer
1Division of Infectious Diseases, Los Angeles Biomedical Research Institute at Harbor, University of California at Los Angeles, Medical Center, Torrance, California, USA. nmishra@labiomed.org
Abstract:
We compared the cell membrane (CM) lipid composition among nine well-characterized daptomycin-susceptible (Dap(s))/Dap-resistant (Dap(r)) methicillin-resistant Staphylococcus aureus (MRSA) strain pairs. Compared to the 9 Dap(s) parental strains, Dap(r) strains (with or without mprF-yycFG mutations) exhibited significantly reduced phosphatidylglycerol (PG) content (P < 0.01), significantly increased total synthesis of lysyl-PG (LPG) (P < 0.01), and reduced carotenoid content (P < 0.05 for 5/9 strains). There were no significant changes in LPG flipping, cardiolipin content, or fatty acid composition among strain pairs.
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