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Updated: May 15, 2026

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

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Published on: May 30, 2012

Itch, an E3 ligase of Oct4, is required for embryonic stem cell self-renewal and pluripotency induction

Bing Liao1, Xiaoming Zhong, Huiming Xu

  • 1Shanghai Stem Cell Institute, Shanghai JiaoTong University School of Medicine, Shanghai, China.

Journal of Cellular Physiology
|December 21, 2012
PubMed

Itch, a C2-WW-HECT domain ubiquitin E3 ligase, plays an important role in various biological processes. However, its role in embryonic stem cells (ESCs) remains unknown. Here, we report that Itch interacts with and targets pluripotency-associated transcription factor Oct4 for ubiquitination. Moreover, Itch enhances Oct4 transcriptional activities and controls Oct4 protein stability dependent on its catalytic activity. Importantly, silencing Itch expression compromises ESC self-renewal capacity and somatic cell reprogramming efficiency. Taken together, our study identifies Itch as a regulator of Oct4 stability and transcriptional activity, establishing a functional link between an E3 ligase and the regulation of pluripotency.

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