Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 15, 2026

A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants
07:26

A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants

Published on: January 1, 2016

Development and evaluation of a new in vivo solid-phase microextraction sampler.

Oluranti P Togunde1, Heather Lord, Ken D Oakes

  • 1Department of Chemistry, University of Waterloo, Ontario, Canada.

Journal of Separation Science
|December 21, 2012
PubMed
Summary

A new in vivo solid-phase microextraction (SPME) device simplifies pharmaceutical residue sampling in fish. This robust tool offers faster, more accessible analysis for environmental monitoring and food safety.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cognitive engagement induces area-specific fingerprints of dopamine, acetylcholine, serotonin, glutamate and GABA in prefrontal cortex and striatum.

bioRxiv : the preprint server for biology·2026
Same author

Nonexhaustive microextraction as a step toward more sustainable chemical analysis in the field and the clinic.

Nature protocols·2026
Same author

<i>In Vivo</i> Negligible Depletion SPME for the Determination of Free and Total Concentrations of Anandamide and 2-Arachidonoylglycerol in the Brain of a Parkinson's Disease Rat Model.

Analytical chemistry·2026
Same author

Defects in auxiliary fuel oxidation and mitochondrial pyruvate transport mark transition to overt heart failure in Tgαq*44 mice.

Journal of translational medicine·2026
Same author

High-throughput screening of per- and polyfluoroalkyl substances in human plasma using biocompatible solid-phase microextraction coupled with mass spectrometry via microfluidic open interface.

Analytica chimica acta·2026
Same author

Comprehensive analysis of exhaled breath VOCs using GC-MS and GC×GC-TOF-MS: a comparative platform evaluation with TFME and NTD sampling for free and total concentrations.

Analytical and bioanalytical chemistry·2025

Area of Science:

  • Environmental Chemistry
  • Analytical Chemistry
  • Ecotoxicology

Background:

  • Solid-phase microextraction (SPME) is a nonlethal technique for in vivo sampling of pharmaceutical residues in fish.
  • Existing SPME methods require improvements in simplicity and robustness for broader laboratory and field applications.

Purpose of the Study:

  • To develop and enhance an SPME device for simplified and robust in vivo sampling of pharmaceutical residues in fish tissue.
  • To demonstrate the practical application of the improved SPME device in field sampling of wild fish.

Main Methods:

  • Development of a novel in vivo SPME device utilizing a polydimethylsiloxane (PDMS) extraction phase.
  • Field sampling of wild fish (Esox masquinongy) using the new SPME device.
  • Analysis of sampled fish tissue using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS).

More Related Videos

Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples
09:42

Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples

Published on: August 7, 2016

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction
09:19

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

Published on: June 1, 2022

Related Experiment Videos

Last Updated: May 15, 2026

A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants
07:26

A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants

Published on: January 1, 2016

Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples
09:42

Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples

Published on: August 7, 2016

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction
09:19

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

Published on: June 1, 2022

Main Results:

  • The new SPME device proved to be a robust and simpler tool for both experts and non-experts compared to traditional devices.
  • Achieved method detection limits in gel and fish tissue ranged from 0.01-0.26 ng/g.
  • Demonstrated acceptable interday reproducibility (8-16% RSD) in homogenized fish tissue.

Conclusions:

  • The developed in vivo SPME device offers a simplified and robust platform for rapid pharmaceutical residue sampling in fish muscle.
  • The device enables precise and accurate detection of target pharmaceuticals like carbamazepine, diazepam, and nordiazepam.
  • This advancement facilitates wider application of nonlethal sampling techniques in environmental monitoring and food safety assessments.