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Coding sequence and flanking regions of the mouse vimentin gene
1Max-Planck-Institut für Zellbiologie, Rosenhof, Ladenburg bei Heidelberg, Federal Republic of Germany.
Molecular & General Genetics : MGG
|March 1, 1990
Summary
Researchers identified the mouse vimentin gene using a cDNA clone and genomic screening. This allowed for the creation of a full-length vimentin clone, revealing extensive sequence homologies.
Area of Science:
- Molecular Biology
- Genetics
- Cell Biology
Background:
- Vimentin is a crucial intermediate filament protein involved in cellular structure and function.
- Understanding vimentin gene regulation and structure is important for various biological processes.
Purpose of the Study:
- To isolate and characterize the mouse vimentin gene.
- To obtain a full-length vimentin cDNA clone for further studies.
Main Methods:
- Screening of a lambda gt11 expression library with a polyclonal antibody to identify a vimentin cDNA clone.
- Screening of an Ehrlich Ascites Tumor cell genomic library using the identified cDNA clone.
- Construction of a full-length cDNA equivalent from the genomic clone.
Main Results:
- Identification of a mouse vimentin cDNA clone.
- Isolation of a genomic clone containing the entire coding sequence and flanking untranslated regions of the mouse vimentin gene.
- Demonstration of extensive homologies between mouse vimentin sequences and those from other species, including the promoter region.
Conclusions:
- The study successfully isolated and characterized the mouse vimentin gene.
- The obtained clone provides a valuable tool for studying vimentin gene expression and regulation.
- Conserved sequences suggest functional importance of these regions across species.