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Published on: February 11, 2020
[Preparation and application of internal surface reversed-phase restricted-access material]
Xiaoyu Wu1, Rong Wang, Hua Xie
1PLA Key Laboratory of the Plateau of the Environmental Damage Control, Lanzhou General Hospital, Lanzhou Command, Lanzhou 730050, China.
Se Pu = Chinese Journal of Chromatography
|December 22, 2012
Summary
A novel internal surface reversed-phase material enables direct analysis of biological samples by excluding large biomolecules while retaining small ones. This method simplifies sample preparation for complex matrices like plasma.
Area of Science:
- Chromatography
- Materials Science
- Analytical Chemistry
Context:
- Direct analysis of complex biological samples like plasma is challenging due to interfering biomacromolecules.
- Existing chromatographic supports often require extensive sample pretreatment steps.
- Restricted-access materials (RAMs) offer a solution by selectively interacting with analytes of interest.
Purpose:
- To develop a convenient synthetic method for a new internal surface reversed-phase restricted-access material (RAM).
- To characterize the structure and evaluate the chromatographic performance of the developed RAM.
- To demonstrate the material's capability for direct analysis of biological samples, such as plasma.
Summary:
- A novel internal surface reversed-phase material was synthesized by functionalizing porous silica with alkyl chains internally and polyvinyl alcohol groups externally.
- Structural characterization confirmed the material's unique surface properties.
- Chromatographic evaluation using various probes and direct plasma sample analysis demonstrated effective protein exclusion and reversed-phase retention, enabling simultaneous biomacromolecule exclusion and micromolecule enrichment.
Impact:
- This RAM facilitates direct injection and analysis of complex biological samples, significantly reducing or eliminating the need for extensive sample preparation.
- It offers a dual functionality of excluding biomacromolecules and enriching micromolecules, improving analytical efficiency.
- The material serves as an effective pretreatment column for online, fast analysis of biological specimens via direct probe inlet, advancing bioanalytical methodologies.
