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Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

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A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

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Published on: May 27, 2014

A systematic approach for developing a robust LC-MS/MS method for bioanalysis.

Min Meng1, Laixin Wang, Troy Voelker

  • 1Tandem Labs, Salt Lake City, UT, USA. min.meng@labcorp.com

Bioanalysis
|December 22, 2012
PubMed
Summary
This summary is machine-generated.

A novel Amoeba™ program streamlines liquid chromatography-tandem mass spectrometry (LC-MS/MS) method development for contract research organizations (CROs). This standardized approach captures critical assay information efficiently, accelerating drug discovery.

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Area of Science:

  • Analytical Chemistry
  • Pharmaceutical Sciences
  • Bioanalysis

Background:

  • Contract research organizations (CROs) face pressure for rapid LC-MS/MS method development in drug discovery.
  • Standardized protocols are needed to efficiently develop and validate bioanalytical methods.

Purpose of the Study:

  • To introduce and detail the Amoeba™ method development program.
  • To demonstrate the program's effectiveness through case studies.
  • To highlight key considerations for robust bioanalytical method development.

Main Methods:

  • Development of a comprehensive, protocol-driven method development program (Amoeba™).
  • Application of the Amoeba™ program across various bioanalytical assays.
  • Analysis of critical parameters such as internal standard response, matrix effects, and carryover.

Main Results:

  • The Amoeba™ program enables efficient capture of critical assay information.
  • Case studies demonstrate successful execution and application of the program.
  • The program facilitates standardized and rapid LC-MS/MS method development.

Conclusions:

  • The Amoeba™ program provides a standardized framework for efficient LC-MS/MS method development.
  • Further evaluation of specific parameters is crucial for robust bioanalytical method refinement.
  • This approach supports the needs of pharmaceutical companies in drug discovery and development.