Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A negative element involved in vimentin gene expression.

F X Farrell1, C M Sax, Z E Zehner

  • 1Department of Biochemistry and Molecular Biophysics, Medical College of Virginia/Virginia Commonwealth University, Richmond 23298.

Molecular and Cellular Biology
|May 1, 1990
PubMed
Summary
This summary is machine-generated.

Researchers identified a novel silencer region in the chicken vimentin gene that represses gene expression. This region binds a 95-kilodalton protein, crucial for regulating tissue-specific vimentin expression during development.

Related Experiment Videos

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

miR-9 Acts as an OncomiR in Prostate Cancer through Multiple Pathways That Drive Tumour Progression and Metastasis.

PloS one·2016
Same author

Perinuclear mRNA localisation by vimentin 3'-untranslated region requires a 100 nucleotide sequence and intermediate filaments.

FEBS letters·2001
Same author

Transketolase gene expression in the cornea is influenced by environmental factors and developmentally controlled events.

Cornea·2000
Same author

Synthesis and erythropoietin receptor binding affinities of N,N-disubstituted amino acids.

Bioorganic & medicinal chemistry letters·2000
Same author

Chromosomal localization of the genes encoding ALDH, BMP-2, R-FABP, IFN-gamma, RXR-gamma, and VIM in chicken by fluorescence in situ hybridization.

Cytogenetics and cell genetics·2000
Same author

The zinc finger repressor, ZBP-89, binds to the silencer element of the human vimentin gene and complexes with the transcriptional activator, Sp1.

The Journal of biological chemistry·2000

Area of Science:

  • Molecular Biology
  • Gene Regulation

Background:

  • Vimentin, an intermediate filament protein, shows tissue- and developmental stage-specific expression.
  • Vimentin is primarily found in cells of mesenchymal origin.
  • Previous studies identified positive regulatory elements (enhancers and promoters) in the chicken vimentin gene.

Purpose of the Study:

  • To identify and characterize negative regulatory elements controlling chicken vimentin gene expression.
  • To investigate the mechanism by which vimentin gene expression is repressed.

Main Methods:

  • Identification of a 40-base-pair silencer region located between enhancer elements.
  • Functional assays using heterologous promoters (herpes simplex virus thymidine kinase).
  • Gel retardation assays and DNase I footprinting to detect protein binding.
  • Southwestern blot analysis to determine protein size.

Main Results:

  • A 40-bp region located at -568 bp acts as a transcriptional silencer.
  • This silencer can repress both the vimentin promoter and a heterologous promoter.
  • The silencer functions independently of position and orientation and shows increased repression with multiple copies.
  • A 95-kilodalton protein binds to this silencer region in HeLa cell nuclear extracts.
  • Protein binding activity correlates inversely with vimentin gene expression during myogenesis.
  • Sequence similarity exists with silencer elements in other genes and species.

Conclusions:

  • A novel silencer element in the chicken vimentin gene binds a 95-kDa protein.
  • This protein-DNA interaction is involved in the negative regulation of vimentin gene expression.
  • The findings contribute to understanding the mechanisms of tissue-specific gene regulation.