Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Aging01:26

Aging

Aging is a complex biological phenomenon influenced by various processes that affect cellular and systemic functions. Several prominent theories attempt to explain its mechanisms, highlighting cellular limitations, oxidative damage, and hormonal changes as central factors in aging.
Cellular Clock Theory
The cellular clock theory posits that the human lifespan is closely tied to the finite capacity of cells to divide, a phenomenon governed by telomeres, which are protective caps at the ends of...
The Effect of Aging on Tissues01:19

The Effect of Aging on Tissues

Several body functions deteriorate with age. The external signs of aging are easily identifiable. For example, the skin becomes dry, less elastic, and thins out, forming wrinkles. The skin of the face begins to appear looser due to a decrease in the levels of elastic and collagen fibers in the connective tissue. Additionally, melanin production in the hair follicle decreases with age, resulting in gray hair. Moreover, the senses of sight and hearing decline, so glasses and hearing aids may...
Tumor Progression02:07

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Consecutive Reactions01:22

Consecutive Reactions

Consecutive reactions involve a sequence where the product of a preceding reaction becomes the reactant for the subsequent one. In a simple scheme, A transforms into B, which further reacts to form C, with rate constants k1 and k2, respectively. This concept is evident in the radioactive decay series. Assuming an initial state with only A present, the conservation of matter leads to three coupled differential equations, determining the concentrations of A, B, and C over time.The rate of change...
Alzheimer Disease l: Introduction01:29

Alzheimer Disease l: Introduction

Alzheimer disease is a chronic, progressive, and irreversible neurodegenerative disorder and the most common cause of dementia in older adults. It leads to gradual neuronal loss, causing cognitive decline, behavioral changes, and loss of functional independence.Risk Factors and EtiologyThe disease is multifactorial. Age is the strongest risk factor, with prevalence doubling every 5 years after age 65. Genetic factors include mutations in genes such as APP, PSEN1, and PSEN2, which are associated...
Cause and Effect01:53

Cause and Effect

While variables are sometimes correlated because one does cause the other, it could also be that some other factor, a confounding variable, is actually causing the systematic movement in our variables of interest. For instance, as sales in ice cream increase, so does the overall rate of crime. Is it possible that indulging in your favorite flavor of ice cream could send you on a crime spree? Or, after committing crime do you think you might decide to treat yourself to a cone?

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Efficacy of traditional Chinese exercises on cognitive function in older adults: a systematic review and meta-analysis of randomised controlled trials.

Age and ageing·2026
Same author

Questions of the future in aging and longevity research at the GIMM Festival.

Nature aging·2026
Same author

Supplements and Drugs Are Associated With Biological Age in a Cohort of Exceptionally Healthy Individuals.

Aging cell·2026
Same author

Obesity and biological aging across the life course: A geroscience framework for metabolic health.

Metabolism: clinical and experimental·2026
Same author

Foundations of Gerophysics.

Aging·2026
Same author

Inferring Gene Regulatory Network Architecture Underlying Complex Traits: An Integrative Analysis of Mutant Lifespan and Gene Expression Profiles Identifies Master Regulators and Key Functional Modules for Yeast Aging.

Aging cell·2026
Same journal

Pitch selectivity in ferret auditory cortex.

Current biology : CB·2026
Same journal

A cell size-dependent competition between geometry and polarity governs nuclear and spindle positioning in early embryos.

Current biology : CB·2026
Same journal

Trophic cascades drive sustainability in the agricultural heritage rice-fish coculture system.

Current biology : CB·2026
Same journal

Tracking Satb2-positive retinal ganglion cells in zebrafish unveils developmental functional reorganization.

Current biology : CB·2026
Same journal

RhoGAP54D promotes cell size asymmetry and inhibits pulsatile myosin activity in Drosophila neural stem cells.

Current biology : CB·2026
Same journal

Increased rates of hybridization in swordtails are associated with water pollution.

Current biology : CB·2026
See all related articles

Related Experiment Video

Updated: May 15, 2026

Using Caenorhabditis elegans for Studying Trans- and Multi-Generational Effects of Toxicants
08:58

Using Caenorhabditis elegans for Studying Trans- and Multi-Generational Effects of Toxicants

Published on: July 29, 2019

Aging: one thing leads to another.

Marion Schmidt1, Brian K Kennedy

  • 1Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA. marion.schmidt@einstein.yu.edu

Current Biology : CB
|December 22, 2012
PubMed
Summary
This summary is machine-generated.

Aging causes mitochondrial decline. In yeast, reduced vacuolar acidification impairs mitochondria by disrupting neutral amino acid transport, revealing a novel aging mechanism.

More Related Videos

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging
09:37

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

Published on: July 14, 2016

Related Experiment Videos

Last Updated: May 15, 2026

Using Caenorhabditis elegans for Studying Trans- and Multi-Generational Effects of Toxicants
08:58

Using Caenorhabditis elegans for Studying Trans- and Multi-Generational Effects of Toxicants

Published on: July 29, 2019

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging
09:37

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

Published on: July 14, 2016

Area of Science:

  • Cellular biology
  • Mitochondrial research
  • Aging mechanisms

Background:

  • Mitochondria are vital for cellular function and are known to deteriorate with age.
  • The specific molecular mechanisms driving age-related mitochondrial decline remain largely unknown.
  • Understanding these mechanisms is crucial for addressing age-associated diseases.

Purpose of the Study:

  • To investigate the underlying causes of age-dependent mitochondrial deterioration in yeast.
  • To elucidate the role of vacuolar function in maintaining mitochondrial health during aging.
  • To identify novel molecular pathways linking aging to mitochondrial dysfunction.

Main Methods:

  • Utilized the yeast model organism (Saccharomyces cerevisiae) to study aging processes.
  • Assessed age-dependent changes in vacuolar acidification using pH-sensitive fluorescent dyes.
  • Quantified mitochondrial function through respiration assays and membrane potential measurements.
  • Investigated the impact of altered vacuolar function on neutral amino acid transport.

Main Results:

  • Demonstrated an age-dependent decrease in vacuolar acidification in yeast cells.
  • Showed that reduced vacuolar acidity leads to impaired mitochondrial function and increased oxidative stress.
  • Identified a critical role for vacuolar neutral amino acid transport in maintaining mitochondrial health.
  • Linked the loss of this transport to mitochondrial dysfunction in aging yeast.

Conclusions:

  • Age-related decline in vacuolar acidification is a key driver of mitochondrial dysfunction.
  • Disruption of vacuolar neutral amino acid transport represents a novel mechanism of mitochondrial aging.
  • These findings provide new insights into the cellular processes underlying aging and offer potential targets for interventions.