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Ultrahigh Resolution Mouse Optical Coherence Tomography to Aid Intraocular Injection in Retinal Gene Therapy Research

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High-sensitivity anastigmatic imaging needle for optical coherence tomography.

Loretta Scolaro¹, Dirk Lorenser, Robert A McLaughlin

¹Optical+Biomedical Engineering Laboratory, School of Electrical, Electronic & Computer Engineering, The University of Western Australia, Crawley, WA 6009, Australia. lscolaro@graduate.uwa.edu.au

|December 22, 2012

Summary

We developed a novel imaging needle for optical coherence tomography (OCT) providing high sensitivity and resolution for in vivo imaging. This compact OCT needle enables detailed superficial and volumetric imaging in biological samples.

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Area of Science:

Biomedical Optics
Medical Imaging Technology

Background:

Conventional free-space optical coherence tomography (OCT) systems face limitations in accessing confined biological tissues.
Miniaturized imaging probes are crucial for in vivo OCT applications, requiring high optical performance and robustness.

Purpose of the Study:

To develop and characterize a high-optical-quality imaging needle for OCT with performance comparable to free-space systems.
To enable sensitive, high-resolution OCT imaging within biological samples using a compact, side-viewing probe.

Main Methods:

A side-viewing needle probe was designed using an angle-polished fiber tip for total internal reflection, encased in a fused glass microcapillary.
An astigmatism-free focusing system exploiting the focal shift phenomenon was implemented to achieve equal working distances.
The needle's performance was evaluated in an aqueous environment, measuring sensitivity and imaging capabilities.

Main Results:

The fabricated OCT needle demonstrated high optical quality with a working distance ratio of 0.98 in an aqueous environment.
The needle achieved a record sensitivity of 112 dB among reported OCT imaging needles.
Successful superficial imaging of human skin and 3D volumetric imaging of a biological sample were demonstrated.

Conclusions:

The developed OCT imaging needle offers a robust and compact solution for high-performance in vivo OCT.
This technology enables sensitive and high-resolution imaging in previously inaccessible or difficult-to-reach biological tissues.
The needle's design represents a significant advancement for minimally invasive OCT applications.