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Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...

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Related Experiment Video

Updated: May 15, 2026

Sample Preparation for Rapid Lipid Analysis in Drosophila Brain Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging
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Sample Preparation for Rapid Lipid Analysis in Drosophila Brain Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging

Published on: July 14, 2022

Biochemical membrane lipidomics during Drosophila development.

Xue Li Guan1, Gianluca Cestra, Guanghou Shui

  • 1Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, 4002 Basel, Switzerland. xueli.guan@unibas.ch

Developmental Cell
|December 25, 2012
PubMed
Summary

This study details lipid profiles across the Drosophila melanogaster life cycle, revealing a constant triacylglycerol ratio during pupation and specific sphingolipid modifications. These findings offer insights into lipid metabolism and gene function.

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Dissection and Lipid Droplet Staining of Oenocytes in Drosophila Larvae
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Dissection and Lipid Droplet Staining of Oenocytes in Drosophila Larvae

Published on: December 28, 2019

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Last Updated: May 15, 2026

Sample Preparation for Rapid Lipid Analysis in Drosophila Brain Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging
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Sample Preparation for Rapid Lipid Analysis in Drosophila Brain Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging

Published on: July 14, 2022

Dissection and Lipid Droplet Staining of Oenocytes in Drosophila Larvae
05:37

Dissection and Lipid Droplet Staining of Oenocytes in Drosophila Larvae

Published on: December 28, 2019

Area of Science:

  • Biochemistry
  • Developmental Biology
  • Genetics

Background:

  • Lipids are essential for cellular functions including energy storage, membrane integrity, and signaling pathways.
  • Understanding lipid dynamics throughout an organism's life cycle is crucial for deciphering complex biological processes.

Purpose of the Study:

  • To comprehensively semiquantify lipids during the entire Drosophila melanogaster life cycle.
  • To gain biological insights into lipid metabolism, regulation, and function through a detailed lipidomic resource.
  • To identify novel enzymes and pathways involved in lipid modification and organismal development.

Main Methods:

  • Liquid chromatography-mass spectrometry (LC-MS) for comprehensive lipid profiling.
  • Analysis of lipid composition across various developmental stages of Drosophila melanogaster.
  • Correlation of lipid changes with gene expression data.

Main Results:

  • A high and consistent triacylglycerol-to-membrane lipid ratio was observed during the pupal stage, irrespective of nutrient intake.
  • Specific alterations in sphingolipid headgroups (glycosylation) and tails (unsaturation, hydroxylation) were identified, correlating with enzyme gene expression.
  • A notable gender bias in phosphoethanolamine-ceramides was detected, suggesting a role in sexual maturation.
  • The gene ghiberti was characterized as a homolog of mammalian serine palmitoyltransferase, essential for male meiotic cytokinesis.

Conclusions:

  • The Drosophila melanogaster lipidome exhibits distinct patterns during development, particularly concerning energy storage and membrane composition.
  • Sphingolipid metabolism is dynamically regulated by specific enzymes, influencing lipid structure and function throughout the life cycle.
  • Lipidomic data provides a valuable resource for understanding gene function, developmental processes, and potential links to sexual maturation and cell division.