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Related Concept Videos

iPS Cell Differentiation01:22

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
Formation of Muscle Fibers from Myoblasts01:13

Formation of Muscle Fibers from Myoblasts

De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription factors...

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Related Experiment Video

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Preparation and Characterization of Graphene-Based 3D Biohybrid Hydrogel Bioink for Peripheral Neuroengineering
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Myoblast differentiation on graphene oxide.

Sook Hee Ku1, Chan Beum Park

  • 1KAIST Institute for the BioCentury, Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.

Biomaterials
|December 25, 2012
PubMed
Summary

Graphene oxide (GO) significantly enhances muscle cell (myogenic) differentiation and myotube formation. This discovery highlights GO

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Nanotechnology

Background:

  • Graphene-based nanomaterials are explored for various biomedical uses.
  • Their impact on myogenic differentiation remains understudied.
  • Graphene oxide (GO) and reduced graphene oxide (rGO) are key materials.

Purpose of the Study:

  • To investigate the effect of GO and rGO on myoblast behavior and myogenic differentiation.
  • To evaluate GO and rGO as substrates for skeletal tissue engineering.

Main Methods:

  • Preparation of GO- and rGO-modified glass substrates.
  • Culturing mouse myoblast C2C12 cells on modified and unmodified substrates.
  • Analysis of cell adhesion, proliferation, and differentiation markers (protein expression, myotube formation, gene expression).

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Main Results:

  • Myogenic differentiation was significantly enhanced on GO substrates.
  • Enhanced differentiation on GO was attributed to serum protein adsorption and nanotopographical cues.
  • GO demonstrated a positive effect on myotube formation and differentiation-specific gene expression.

Conclusions:

  • Graphene oxide effectively stimulates myogenic differentiation.
  • GO shows potential as a biomaterial for skeletal tissue engineering applications.
  • The study elucidates mechanisms underlying GO's pro-myogenic effects.