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A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Stereoselective separation of spiroindoline phytoalexins on R-naphthylethyl cyclofructan 6-based chiral stationary
Ta'ána Gondová1, Ján Petrovaj, Peter Kutschy
1Department of Analytical Chemistry, Faculty of Science, P.J. Šafárik University, Moyzesova 11, SK-040 01 Košice, Slovak Republic. tatana.gondova@upjs.sk
Abstract:
In this study, the recently developed type of chiral stationary phase, R-naphthylethyl-derivatized cyclofructan 6 (RN-CF6) was used for direct enantioseparation of novel chiral analogs of spiroindoline phytoalexins with potential anticancer and antimicrobial activity using HPLC. The experiments were performed under normal phase elution. Effects of polar modifier, the structure of the analytes and temperature on the separation were investigated. The thermodynamic parameters were evaluated from van't Hoff plots. Cyclofructan-based chiral stationary phase, RN-CF6, was able to separate all eighteen racemic mixtures of studied phytoalexins including cis- and trans-diastereoisomers.
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