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Related Experiment Video

Updated: May 15, 2026

Correlative Light Electron Microscopy (CLEM) for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells

Correlative Light Electron Microscopy (CLEM) for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells

Published on: September 7, 2018

Localization of fluorescently labeled structures in frozen-hydrated samples using integrated light electron

F G A Faas¹, M Bárcena, A V Agronskaia

¹Department of Molecular Cell Biology, Section Electron Microscopy, Leiden University Medical Center, P.O. Box 9600, 2300 RC, Leiden, The Netherlands.

Journal of Structural Biology

|December 25, 2012

Summary

Cryogenic integrated light and electron microscopy preserves native biological states for high-resolution imaging. This advanced technique enhances fluorescence signal and reduces photobleaching, improving correlative microscopy success.

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Area of Science:

Biological imaging
Microscopy techniques

Background:

Correlative light and electron microscopy (CLEM) integrates fluorescence and electron microscopy for multi-resolution biological studies.
Previous integrated CLEM approaches operated at room temperature, posing challenges for hydrated specimens and fluorescence signal integrity.

Purpose of the Study:

To present an integrated approach for correlative microscopy under cryogenic conditions.
To highlight the advantages of cryo-CLEM over room temperature methods for biological sample analysis.

Main Methods:

Development of an integrated light and electron microscope system operating at cryogenic temperatures.
Application of the cryo-integrated system to various biological samples, including bacteria, microtubules, fibroblasts, and mammalian cells.

Main Results:

Cryo-integrated CLEM preserves the native hydrated state of biological specimens.
Maintained fluorescence signal integrity without quenching from heavy atom stains.
Demonstrated reduced photobleaching compared to room temperature methods.
Successfully applied to diverse biological applications, including bacteria detection and subcellular structure analysis.

Conclusions:

Cryo-integrated light and electron microscopy offers significant advantages for high-resolution imaging of biological systems.
This technique is crucial for preserving sample integrity and fluorescence signals, enabling more accurate morphological and structural studies.